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FAQ

Frequently Asked Questions

  • Is custom media available for purchase?

Yes. Custom media will be charged at the same price as the original media. Please inquire if we can perform this request.

  • Do you provide training sessions?

Yes. There are several one to three-day training sessions held each year. For best practice please inquire via email for the next available session. Topics include all cell culture protocols that involve general cell maintenance including isolating human airway epithelial cells, counting, seeding, and freezing cells. There are links for some of these training handouts under the category: Protocols and Research Applications.

  • What is a chartfield string entry? Who do I contact with chartfield string entry questions?

Here is a webpage entirely dedicated to the explanation of chartfield string entry: http://unclabmanual.web.unc.edu/chartfield-string/.

  • What is the quality assurance/quality control of the cells being purchased?

Our rigor and reproducibility page best describes the details of our scientific process. However, a basic summary of our QC protocols includes a mycobacterial analysis of media, an Ussing which tests the resistance of the cells, histology images studied, and a DNA and RNA protein analysis.

  • How should we measure CO2 in our incubators?

It is best to measure CO2 levels with fyrite. Here is an instructional video on how to check fyrite:
https://www.youtube.com/watch?v=_K-L758UKqU
If you have more updated technology such as the Vaisala GM70:
https://www.youtube.com/watch?v=xl8d2bD1Dfc

  • I am having trouble with iLabs, what can I do?

Here is the iLabs FAQ page:
https://help.ilab.agilent.com/35322-getting-started/299372-welcome-to-ilab-help
Additionally, they are very quick at responding when contacted. Their support page can be found below:
https://help.ilab.agilent.com/35322-getting-started/296444-support

  • How should I cite the Randell Lab for their contributions to my research?

For any effort we provide to your scientific work please recognize the MLI Tissue Procurement and Cell Culture Core at UNC as follows in publications: the Cystic Fibrosis Foundation at UNC: CFFBOUCHE19R0 and cite the NIH CFRTCC Cell Models Core at UNC: NIH P30DK065988.