The AA Core is excited to announce single-cell Western blot service using the Protein Simple Milo! The Milo platform measures expression of multiple proteins across 1,000+ single cells in just 4-6 hours.
The CGIBD’s Advanced Analytics (AA) Core provides high-quality, quantitative solutions for studying proteins and nucleic acids at the bulk or single-cell level. Our service offerings include:
- Immunoassays & plate reading (ELISA & bead-based multiplex)
- High-throughput qPCR (Fluidigm BioMark HD)
- Single-cell Genomics (Fluidigm C1, droplet-based RNASeq)
- Single-cell Protein Detection & Quantification (Protein Simple Milo)
The AA Core also provides ancillary FACS isolation, sequencing, and data analysis services as part of our “turn-key” solutions to your genomics and proteomics needs. Feel free to contact Carlton Anderson (Core Manager) or check out the CGIBD Core Webpage for more information regarding our technologies, instruments, and workflows.
HIGHLIGHTS:
The AA Core is excited to announce single-cell Western blot service using the Protein Simple Milo! The Milo platform measures expression of multiple proteins across 1,000+ single cells in just 4-6 hours. Milo captures cells on a novel “scWest chip” (Fig. 1), performs SDS-PAGE, and immobilizes proteins prior to antibody probing and fluorescence imaging. Loaded chips can be stored, stripped, and re-probed multiple times within a 9-month period. Milo is a powerful tool both alone and in combination with the Core’s other single cell genomics services (Fig. 2). More details regarding this promising new technology can be found on the Core Webpage.
Figure 1. A Protein Simple Milo scWest chip. An example of a typical fluorescence readout from two probed proteins is shown. Image Source: https://www.proteinsimple.com/milo.html.
Figure 2. Overview of simultaneous scRNA-Seq and scWest validation using the Protein Simple Milo platform. Image Source: https://www.proteinsimple.com/milo.html.
The AA Core’s mission is to support research into the etiology and pathogenesis of disease by providing a range of full-service, cost-effective immunoassay, genomics, and gene expression assays to the scientific community. To set up an expert consultation or begin a project with the AA Core, please contact the Core Manager.