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Center for Structural Biology

References

Solid Phase Peptide Synthesis

1. “Fmoc Solid Phase Peptide Synthesis. A Practical Approach” Edited by W. Chan and P. White, Oxford University Press (2000).

2. I. Coin, M. Beyermann, M. Biener “Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences” Nature Protocols (2007), 2, 3247 – 3256.

Selected Publications Involving Core Facility

  1. Matthew R Marunde, et. all, “Nucleosome context regulates chromatin reader preference” Nucleic Acids Research (2025), 53, 20, gkaf1061.
  2. Y. Zhao, A. Nguyen, E. C. Arteaga, A. Skrajna, K. Krajewski, D. Goldfarb, R. K McGinty, “Post-translational modification of H2B C-terminal helix regulates nucleosome interactions and chromatin signaling” Nucleic Acids Research (2025) 53,17, gkaf897.
  3. K. Jain, P.-E. Kougnassoukou Tchara, A. Khan, A. l. B. Mengistalem, A. P. Holland, C. N. Bowman, M. R. Marunde, T. A. Engel, I. K. Popova, S. W. Cooke, K. Krajewski, M.-C. Keogh, J.-P. Lambert, B. D. Strahl “Histone H3 N-terminal recognition by the PHD finger of PHRF1 is required for proper DNA damage response” Nucleic Acids Research (2025) 53, 13, gkaf666.
  4. M. R. Marunde, H. A. Fuchs, J. M. Burg, I. K. Popova, A. Vaidya, N. W. Hall, E. N. Weinzapfel, M. J Meiners, R. Watson, Z. B. Gillespie, H. F. Taylor, L. Mukhsinova, U. C Onuoha, S. A Howard, K. Novitzky, E. T McAnarney, K. Krajewski, M. W Cowles, M. A Cheek, Z.-W. Sun, B. J Venters, M.-C. Keogh, C. A Musselman, “Nucleosome conformation dictates the histone code”
    eLife 13:e78866     (2024).
  5. C. S. Anyetei-Anum, M. P. Leatham-Jensen, G. C. Fox, B. R. Smith, V. R. Chirasani, K. Krajewski, B. D. Strahl, J. M. Dowen, A. G. Matera, R. J. Duronio, D. J. McKay “Evidence for dual roles of histone H3 lysine 4 in antagonizing Polycomb group function and promoting target gene expression” Genes & development (2024) 38 (21-24), 1033–1046.
  6. S. B. Rothbart, K. Krajewski, B. D. Strahl, S. M. Fuchs “Peptide microarrays to interrogate the histone code” Methods in Enzymology (2012) 12, 107-135.
  7. S.M. Fuchs, K. Krajewski, V. Miller, R.W Baker, B.D. Strahl “Influence of combinatorial histone modifications on antibody and effector protein recognition” Current Biology (2011) 11, 53-58.
  8. S. B. Rothbart, K. Krajewski, N. Nady, W. Tempel, S Xue, A.I. Badeaux, D. Barsyte-Lovejoy, J. Y. Martinez, M. T. Bedford, S.M. Fuchs, B. D. Strahl “Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation” Nature Structural & Molecular Biology (2012) 19, 1155-1160.

Antibodies and MAP’s

6. J.P. Tam, J.C. Spetzler “Multiple Antigen Peptide System” Methods in Enzymology (1997), 289, 612-636.

7. J.P. Briand, C. Barin, M.H. van Regenmortel, S. Muller “Application and limitations of the multiple antigen peptide (MAP) system in the production and evaluation of anti-peptide and anti-protein antibodies” J. Immunol. Methods (1992), 156, 255-265.