QAQC Clean Up Methods
My QAQC results show one of these failures…what do I do?
HTSF will discuss with you the best way to resolve an issue after we run QAQC.
RNA Clean Up Methods
| Clean Up Method | Resolution for the following Issues |
| EtOH cleanup | degraded samples |
| RNA XP bead cleanup | good for transferring samples if not supplied in DNase/RNase-Free H2O OR potential contamination of the sample |
| RNA Clean and Concentrator (Zymo research) | Column cleanup; use to DNase treat RNA samples |
| Pippin Prep Size Selection (gel size selection) | best for high resolution bp selection; preferred for small RNA cleanup which has primer dimers at 140-160 bp and a library peak at ~180 bp
|
DNA Clean Up Methods
| Clean Up Method | Resolution for the following Issues |
| 2x Beadwash | good for maximum recovery of sample and to transfer samples from improper submission solution (ex: PCR buffer)
*good for any type of dsDNA material* |
| 0.8x Beadwash |
aggressive beadwash to remove primer dimers in the 100-160 bp range when library size is over 300 bp |
| 1.0x Beadwash |
less aggressive beadwash to remove primer dimers in the 100-160 bp range when library size is ~250 bp
|
| SPRI (two-sided beadwash) |
good for general size selection between 160 bp and 600 bp
|
| Pippin Prep Size Selection (gel size selection) |
best for high resolution bp selection; preferred for small RNA cleanup which has primer dimers at 140-160 bp and a library peak at ~180 bp
|