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ASI diSPIM Light Sheet
The dual Selective Plane Illumination Microscope for cleared tissue (ct-dSPIM) is a light sheet microscope configuration that allows for dual views of large samples and is suitable for fast and gentle imaging. Both objectives have a light sheet scanner and camera and the two datasets can be merged to produce an image with isotropic resolution.

Location: 5114 MEJ

Training and Usage: 

Details coming soon!

Acknowledgements

We suggest using the following language if the ASI ct-dSPIM was used in acquiring your images and data:

“The ASI ct-dSPIM microscope was funded with support from NIH grant S10 MH130455.”

Specifications:

Objectives

MagnificationNAWD (mm)Immersion Media
0.4 @ 1.45 RI12Multi-immersion
0.7 @ 1.45 RI10Multi-immersion

Lasers

Oxxius 488, 561, 638, 751 nm

Detectors

ORCA Fusion back-thinned Gen-III sCMOS camera

Software

Micro-Manager