An inverted widefield microscope with automated scanning for large area real-time mosaic imaging and High Content (HC) acquisition and analysis. Controlled by Nikon Elements software capable of intelligent real-time integration of analysis and acquisition workflows and the High Content Analysis (HCA) module for advanced acquisition and analysis.
Location: 5114 MEJ
Modalities
- Color/Brightfield/Fluorescence Widefield Imaging
- Mosaic Imaging
- HCA Imaging and Analysis
Specifications
Objectives
| Magnification | NA | WD (mm) | Correction | Immersion | Availability |
| 4X | 0.13 | 17.2 | Plan Fluor | Air | |
| 4X | 0.2 | 20 | Plan Apochromat | Air | |
| 10X | 0.3 | 16 | Plan Fluor | Air | |
| 10X | 0.45 | 4 | Plan Apochromat | Air | |
| 20X | 0.5 | 2.1 | Plan Fluor | Air | |
| 20X | 0.7 | 1.3-2.3 | Plan Fluor | Air | |
| 40X | 0.75 | 0.66 | Plan Fluor | Air | |
| 40X | 0.95 | 0.95 | Plan Apochromat | Air | |
Filter Sets
| Position | Dyes | Excitation (nm) | Emission (nm) | Notes |
| 1 | DAPI | 325-375 | 435-485 | |
| 2 | GFP | 450-490 | 500-550 | |
| 3 | TxRed, mCherry | 520-580 | 592.5 – 667.5 | |
| 4 | Narrow Cy5 | 625 – 655 | 665 – 715 | |
| 5 | Broad Cy5 | 590 – 650 | 667.5 – 737.5 | |
| 6 | Cy7 | | | Available Upon Request |
Detectors
| Camera | Specs |
| Fluorescence | pco.edge 4.2Q High QE sCMOS camera |
| Color | Nikon DS-Fi3 CMOS Color camera |
Software
Filter Spectra
Coming Soon!