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Student Thesis Defense: Justin Rectenwald (Pearce and Frye labs)
November 5, 2020 @ 10:00 am - 11:00 am
Biochemistry and Biophysics Student Defense:
Student:
Justin Rectenwald (student of Ken Pearce and Stephen Frye)
Title:
“Technology Development for Inhibitor Discovery of Chromatin Associated Proteins Linked to Lysine Methylation”
Date/location:
Thursday, November 5, 2020 at 10:00am via Zoom meeting.
Links will be provided to UNC affiliated email addresses only or through written consent of defending student.
Abstract
The identification of molecules that tightly bind to therapeutically relevant
targets remains one of the biggest challenges in biomedical research.
Continued development of discovery technologies that are cost effective,
fast, reliable, and reproducible are essential for the generation of new tool
compounds and potential therapeutics. A target-class driven strategy for
discovery of potent and selective inhibitors is an approach adopted by
many academic and industrial labs. Development of multiple,
complementary strategies for hit discovery and characterization suitable
for target-class approaches can greatly enhance the speed and
dependability of biomedical research. Herein, is the description of a series
of technologies developed and applied to chromatin associated proteins
involved in either the recognition or placement of methyl groups upon
lysine residues of histone tails. A generalized TR-FRET assay and panel, a
label-free mass-spectrometry catalytic assay, and a focused DNA-Encoded
Library platform and their applications are summarized.
The identification of molecules that tightly bind to therapeutically relevant
targets remains one of the biggest challenges in biomedical research.
Continued development of discovery technologies that are cost effective,
fast, reliable, and reproducible are essential for the generation of new tool
compounds and potential therapeutics. A target-class driven strategy for
discovery of potent and selective inhibitors is an approach adopted by
many academic and industrial labs. Development of multiple,
complementary strategies for hit discovery and characterization suitable
for target-class approaches can greatly enhance the speed and
dependability of biomedical research. Herein, is the description of a series
of technologies developed and applied to chromatin associated proteins
involved in either the recognition or placement of methyl groups upon
lysine residues of histone tails. A generalized TR-FRET assay and panel, a
label-free mass-spectrometry catalytic assay, and a focused DNA-Encoded
Library platform and their applications are summarized.
