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Protein Expression and Purification

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Protein Expression and Purification

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Nathan Nicely, PhD

Nathan Nicely, PhD

Director - UNC Macromolecular Crystallography
Protein Expression and Purification

Email: nathan.nicely@unc.edu
Telephone: (919) 843-7174

Office: 124 Medical Research Bldg. B

Mailing Address:
CB#7365, 4009 Genetic Medicine Bldg,
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-7365

Shipping Address:
150 Bragg Dr.
Medical Research Bldg. B
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-7365

To make an inquiry, please complete the form below:

Protein production inquiry

Name*
Email address*
Institution/company*
Target protein*
Expression system*
- HEK/CHO
- Hybridoma
- E coli
- Other
- Don't know
Do you have an expression plasmid bearing the gene?*
- Yes and can provide a starting quantity
- Yes and can provide endotoxin-free mg quantities
- No, we will need it constructed
- For hybridoma expression - a stable line or selected pool
Have you made this protein previously yourself?*
- Yes
- No
What is the expected yield (mg per L culture)?*
- Expresses well - ~10+ mg/L depending on system
- Expresses moderately - ~1 mg/L
- Expresses poorly - <1 mg/L
- Don't know
- N/A - we only want the pellet or medium
Is the protein soluble in aqueous solution?*
- Yes
- Soluble with detergent
- No - poorly soluble / needs organic solvent or heavy detergent
- N/A - we only want the pellet or medium
Additional info including any published articles with methods*

Δ

You may also contact us at pepcore@med.unc.edu but please include responses to all the above questions.

Mailing Address:
CB#7380, 4009 Genetic Medicine Bldg
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-7365

Shipping Address:
Medical Research Bldg. B
150 Bragg Drive
Chapel Hill, NC 27514

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Contact Us: pepcore@med.unc.edu

Web Issues? Email: bclarke@med.unc.edu

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