The facility offers a full range of protein expression and purification (PEP) services. Outlined below is a general description of the services and capabilities that we offer. For more detailed information go to the individual service pages for Expression, Protein Purification, and Training, Expertise and Consultation.

We are co-localized with the macromolecular x-ray crystallography (MX) core, so modifying your project to include crystallography is really easy. We, however, do not offer any standardized, fixed price services like the MX core; all PEP projects present their own unique set of challenges and goals. Each project will be billed as close as possible to actual costs (non-profit, as we are a recharge center); this will include all consumables and staff time required.

We also allow and encourage users with prior experience to use the facilities autonomously. To do so, users must undergo training and/or be certified as an independent operator by our staff. Training time typically varies with each user and activity.

Our primary mission is to provide services to UNC users, but we also offer our services to external users including non-UNC academic users and commercial companies.

Expression Services

  • E. coli Expression
  • Baculovirus-Mediated Insect Cell Expression
  • Mammalian Cell Expression

Protein Purification

  • Fast Protein Liquid Chromatography (FPLC)
    • AKTAxpress
    • AKTA FPLC
  • Tangential Flow Filtration (TFF)

Training, Expertise, and Consultation

We have decades of combined experience to bring to bear on your research aims.

Fees

We have a cost recovery structure designed to allow for a wide array of workflows and special project needs. Example basic package estimates are provided following this link.

A special note about antibody production

We do not have the capability to generate polyclonal antibodies against specific targets, nor can we isolate monoclonals from polyclonals. However, we routinely express antibodies recombinantly in HEK culture via transient co-transfection and in client-provided hybridoma cell lines.