Protein Expression and Purification
The Protein Expression and Purification (PEP) core specializes in the production of pure, functional proteins for structural, biophysical, and biochemical studies. It is specifically designed as a “front-end” interface to other components of the Center for Structural Biology. However, the ability to produce and purify milligram amount of protein enables a broad array of applications and research that extend far beyond the bounds of structural biology, as protein reagents are essential to most areas of biomedical research.
The facility offers three categories of service:
The PEP Core is happy to do work for researchers at UNC as well as those at non-UNC academic institutions and commercial companies.
Our offerings and strengths include the following:
- Recombinant protein expression in HEK, CHO, hybridoma, and bacterial cells.
- Numerous chromatography methods available for purification.
- High production scales for immunizations, drug discovery, and structural biology.
- Low endotoxin protein production.
- Custom packages to efficiently suit your needs.
We obtain on-going support from the UNC Lineberger Comprehensive Cancer Center through the University Cancer Research Fund and the Cancer Center Support Grant. Consequently, publications supported by the UNC Center for Structural Biology must acknowledge NIH grant P30CA016086 and be submitted to PubMed Central in compliance with the NIH Public Access Policy.
Suggested acknowledgement: “This work was performed in the UNC Protein Expression and Purification & Macromolecular Crystallography core lab supported by the National Cancer Institute of the National Institutes of Health under award number P30CA016086. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.”