Key words: mouse models for human diseases, genes that modify disease progression
Our lab is using the recently developed technique known as gene targeting to create animal mouse models for human diseases and to identify genes that modify the progress of these diseases. The technique of gene targeting, which allows us to introduce specific changes into the genomes of experimental animals, relies on recent advances in the fields of homologous recombination and embryonic stem (ES) cell technology. The first step in this procedure, once the gene to be modified has been chosen, is to obtain corresponding genomic DNA sequences using stardard cloning techniques. After introducing desired mutations into the cloned DNA using conventinal molecular biology techniques, the genomic sequences are incorporated into a plasmid known as a targeting vector. When this plasmid is introduced into mouse ES cells, the altered genomic sequences are able to undergo homologous recombination with the corresponding endogenous sequences, thereby inroducing the desired modifications into the moust genome. ES cells are unique in that they can be grown in tissue culture while maintaining their totipotency. Thus, one ES cells carrying the desired mutation are identified, they can be returned to an embryo, resulting in the formation of a chimeric animal. If the embryonic stem cells participate in the formation of the germ line of the chimeric animal. the mutation will be transmitted to the chimera’s offspring, and a mouse line carrying the desired mutation will be created. The effect of this alteration can then be studied in vivo. This technology has opened an exciting new avenue for addressing numerous biological and medical problems.
We have used gene targeting to generate an animal model for the most common genetic disease n the Caucasian population, cystic fibrosis. We are continuing to characterize this animal and to modify it to produce a disease that more closely resembles human cystic fibrosis. A second area in which our lab is interested involves the study of the inflammatory processes involved in allergic responses, asthma, and arthritis. Our current efforts are aimed at generating animals deficient in various factors that are believed to be important in these diseases. By providing us with a better understanding of the immunological processes that underlie allergic responses, asthma and arthritis, these animals should help us to identify more effective treatments for these diseases.
- SOM DEI Program part 1 – Unconscious Bias
- Martina Kovarova, Research Assistant Professor, Email
- Jay Snouwaert, Research Associate, Email
- Rachel Lail, Graduate Student, Email
- Anne Latour, Technician. Email
- Mytrang Nguyen, Technician, Email
- Zhidan Xiang, Postdoctoral fellow, Email
- Michael Backlund, Postdoctoral fellow, Email
- Steven Lommatzch, MD, Pulmonary fellow, Email
- Sarah Keilson, Undergraduate student, Email
Beverly Koller in UNC Genetics News
November 29, 2021
Department of Genetics Publications November 14th – 27th 2021
Department of Genetics faculty, postdocs, students and collaborators published 16 papers during November 14th – 27th 2021.
November 15, 2021
Department of Genetics Publications October 31st – November 13th 2021
Department of Genetics faculty, postdocs, students and collaborators published 12 papers during October 31st – November 13th 2021. A multi-omic single-cell landscape of human gynecologic malignancies. Regner MJ, Wisniewska K, Garcia-Recio S, Thennavan A, Mendez-Giraldez R, Malladi VS, Hawkins G, Parker JS, Perou CM, Bae-Jump VL, Franco HL. Mol Cell. 2021 Nov 1:S1097-2765(21)00842-X. doi: …
October 14, 2021
Koller award R21 from NIAID
Bev Koller, PhD, Associate Professor of Genetics, was awarded a new R21 from the National Institute of Allergy and Infectious Diseases (NIAID) titled “Mouse models for study of the NLRP1 and CARD8 inflammasomes”.
September 16, 2021
Ting and Koller awarded R56 from NIAID to study role of inflammasomes in COVID-19 infection
Jenny Ting, PhD (Distinguished Professor, Department of Genetics, Department of Microbiology & Immunology, LCCC) was awarded a R56 grant titled “Role and Mitigation of Inflammasomes and Inflammation During COVID-19” from the National Institute of Allergy and Infectious Diseases (NIAID).
September 9, 2021
Koller and Styblo awarded new R01 from NIEHS
Mirek Styblo (PhD, Professor, Department of Nutrition) and Bev Koller (PhD, Associate Professor, Department of Genetics) are Co-PIs on a new R01 titled “Humanized mouse models for arsenic toxicology” from the National Institute of Environmental Health Sciences (NIEHS).
September 9, 2021
Won, Mohlke, Love awarded UM1 as part of the new IGVF consortium at NHGRI
Hyejung Won (PhD, Assistant Professor, Department of Genetics, Neuroscience Center), Karen Mohlke (PhD, Professor, Department of Genetics), and Mike Love (PhD, Assistant Professor, Department of Biostatistics, Department of Genetics), were awarded a 5-year $9.25 million UM1 grant from the National Human Genome Research Institute (NHGRI) titled “Systematic in vivo characterization of disease-associated regulatory variants”. The …
September 7, 2021
Department of Genetics Publications August 22nd – September 4th 2021
Department of Genetics faculty, postdocs, students and collaborators published 9 papers during August 22nd – September 4th 2021. SnapHiC: a computational pipeline to identify chromatin loops from single-cell Hi-C data. Yu M, Abnousi A, Zhang Y, Li G, Lee L, Chen Z, Fang R, Lagler TM, Yang Y, Wen J, Sun Q, Li Y, …
May 29, 2021
Department of Genetics Publications for May 16-29, 2021
Department of Genetics faculty, postdocs, students and collaborators published nineteen papers during May 16-29, 2021.
May 17, 2021
Department of Genetics Publications for May 2-15, 2021
Department of Genetics faculty, postdocs, students and collaborators published twelve papers during May 2-15, 2021.
January 4, 2021
Department of Genetics Publications for December 13-26, 2020
Department of Genetics faculty, postdocs, students and collaborators published nine papers during Dec. 13-26, 2020.