Xian Chen

Research: Systems cancer biology and immunology, cancer therapeutic response, protein-protein interaction networks (interactomes), post-translational modifications, epigenetic regulation, signal transduction, disease marker discovery.

Xian Chen

Associate Professor of Biochemistry and Biophysics
Faculty Director, Quantitative Proteomics Center for Disease Marker Discovery
(PhD - Penn State University)

ACCEPTING STUDENTS

120 Mason Farm Road, CB# 7260
3072 Genetic Medicine
Chapel Hill, NC 27599-7260
919-843-5310

Chen Lab Website

HONORS & AWARDS

  • Presidential Early Career Award for Scientists and Engineers (PECASE), 1999
  • Department of Energy Early Career Award for Scientists and Engineers, 1999
  • The 2001 Patent & Licensing Awards, Los Alamos National Lab, 2002

RESEARCH

Our research focuses on both developments and applications of unconventional and transformative technology of systems biology, novel mass spectrometry-based proteomics and proteogenomics approaches in particular, to elucidate the molecular mechanisms underlying pathogenesis of various human diseases such as cancer, asthma, and immune disorders. We are interested in a broad range of signaling and epigenetic regulatory pathways/mechanisms underlying exactly how tumor cells escape from immune surveillance, but not limited to the Toll-like receptor (TLR)-mediated pathways and DNA damage response pathways. Our ultimate goal is to mechanistically derive novel, precise disease markers for early diagnosis and therapeutic intervention.

As the Director of Technology Development in UNC Proteomics Center, Dr. Chen has an innovative track record in developing an array of new proteomic and proteogenomic technologies for both discovery and clinical application in cancer biology and immunology. He is also the UNC PI of the NCI Clinical Proteomic Tumor Analysis Consortium (CPTAC). He has developed many novel MS-based quantitative proteomic methods; he invented amino acid-coded mass tagging (AACT) (a.k.a SILAC) that has unique strengths for comparative analysis of protein-protein interactions and post-translational protein modification. The Chen lab has demonstrated the unique strength of quantitative proteomics in dissecting and discovering new pathways involved in immune/inflammatory signaling and cancer epigenetic regulation.

PUBLICATIONS pubmed.png (click for Full Publication List)

  • Li Wang, Ling Xie, YuanYu Lee, Srinivas Ramachandran, Li Zhou, Zhen Yan, Krzysztof Krajewski, Feng Liu, Brian D. Strahl, Jian Jin, Nikolay V. Dokholyan, and Xian Chen* (2015) A non-canonical bromodomain module promotes DNA damage response and radioresistance through recognizing a radiation-inducible lysine acetylation. Chemistry & Biology (Cell Press), In press.
  • Cui Liu, Yanbao Yu, Feng Liu, John A. Wrobel, Li Zhou, Xin Wei, Harsha P. Gunawardena, Jian Jin, andXian Chen* (2014) A novel chromatin-activity-based protein complex pull-down approach reveals a chromatin complexome for systems regulation of gene-specific silencing. Nature Communications, 5, 5733, DOI:10.1038/ncomms6733
  • Ling Xie, Cui Liu, Li Wang, Harsha P. Gunawardena, Yanbao Yu, Ruyun Du, Debra J. Taxman, Penggao Dai, Zhen Yan, Jing Yu, Stephen P. Holly, Leslie V. Parise, Yisong Wan, Jenny P. Ting, and Xian Chen* (2013) Protein Phosphatase 2A Catalytic Subunit a (PP2Ac) Plays a MyD88-dependent, Central Role in the Gene-Specific Regulation of Endotoxin Tolerance (ET). Cell Reports3(3) 678-688.
  • Siwei Tang, Huimin Bao, Yang Zhang, Jun Yao, Pengyuan Yang, and Xian Chen* (2013) “14-3-3ε Mediates the Cell Fate Decision-making Pathways in Response of Hepatocellular Carcinoma to Bleomycin-induced DNA Damage” PLoS One, 8(3), e55268.
  • YuanYu Lee, YanBao Yu, Harsha P. Gunawardena, Ling Xie, and Xian Chen* (2012) BCLAF1 is a Radiation-induced H2AX-interacting Partner Involved in gH2AX-mediated Regulation of Apoptosis and DNA Repair, Cell Death & Disease (Nature Publishing Group), 3, e359.
  • Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, Tanzer A, Lagarde J, Lin W, Schlesinger F, Xue C, Marinov GK, Khatun J, Williams BA, Zaleski C, Rozowsky J, Röder M, Kokocinski F, Abdelhamid RF, Alioto T, Antoshechkin I, Baer MT, Bar NS, Batut P, Bell K, Bell I, Chakrabortty S, Chen X, Chrast J, Curado J, Derrien T, Drenkow J, Dumais E, Dumais J, Duttagupta R, Falconnet E, Fastuca M, Fejes-Toth K, Ferreira P, Foissac S, Fullwood MJ, Gao H, Gonzalez D, Gordon A, Gunawardena H, Howald C, Jha S, Johnson R, Kapranov P, King B, Kingswood C, Luo OJ, Park E, Persaud K, Preall JB, Ribeca P, Risk B, Robyr D, Sammeth M, Schaffer L, See LH, Shahab A, Skancke J, Suzuki AM, Takahashi H, Tilgner H, Trout D, Walters N, Wang H, Wrobel J, Yu Y, Ruan X, Hayashizaki Y, Harrow J, Gerstein M, Hubbard T, Reymond A, Antonarakis SE, Hannon G, Giddings MC, Ruan Y, Wold B, Carninci P, Guigó R, Gingeras TR. (2012) Landscape of transcription in human cells, Nature, 489, 101-108.
  • Yu Lei, Haitao Wen,Yanbao Yu, Debra J. Taxman, Lu Zhang, Douglas G. Widman, Karen V. Swanson, Kwun-Wah Wen, Blossom A. Damania, Chris B. Moore, Patrick M. Giguère, David P. Siderovski, John Hiscott, Babak Razani, Clay F. Semenkovich, Xian Chen, and Jenny P.-Y. Ting (2012) NLRX1 and TUFM form a mitochondrial complex that regulates type 1 interferon and autophagy.Immunity, 36, 933-46.
  • ENCODE Project Consortium, Bernstein BE, Birney E, Dunham I, Green ED, Gunter C, Snyder M. (2012) An Integrated Encyclopedia of DNA Elements in the Human Genome. Nature, 489, 57-74.
  • Yanbao Yu, Ling Xie, Harsha P. Gunawardena, Jainab Khatun, Christopher Maier, Maarten Leerkes, Morgan Giddings, and Xian Chen* (2012) GOFASP: An Integrated Approach of GELFrEE, Optimized Digestion-assisted FASP for Efficient and Comprehensive Membrane Proteome Analysis. Anal. Chem, 84, 9008-9014.
  • Harsha P. Gunawardena, Yi Huang, Haiyang Wang, Ling Xie, Ruyun Du, and Xian Chen (2011) Unambiguous Identification of Serine Phosphorylations of LRR Fli-I-interacting protein 2 involved in TLR4-Mediated Signaling. Journal of Biological Chemistry,286, 10897-10910.
  • Shimin Zhao, Wei Xu, Wenqing Jiang, Wei Yu, Yan Lin, Tengfei Zhang, Jun Yao, Li Zhou, Hong Li, Yixue Li, Jiong Shi, Lunxiu Qin, Pengyuan Yang, Xian Chen, Qunying Lei, Kun-Liang Guan*, and Xue Xiong* (2010) Protein acetylation plays an extensive role in regulating cellular metabolism, Science, 327, 1000-1004.
  • Ling Xie, Linhong Jing, Yanbao Yu, Kazuhiro Nakamura, Carol E. Parker, Gary L. Johnson, and Xian Chen* (2009) In vivo Profiling Endogenous Interactions with Knock-out (iPEIK), Anal Chem81 (4),1411–1417.
  • Peigao Dai, Sun Young Yeong, Yanbo Yu, Weidong Wu, Taohua Leng, Ling Xie, and Xian Chen* (2009) Modulation of TLR Signaling by Multiple MyD88-Interacting Partners Including Leucine-Rich Repeat Fli-I-Interacting Proteins1, Journal of Immunology, 2009, 182, 3450-3460.
  • Xian Chen*, Liwei Sun, Yubao Yu, Yue Yan, and Pengyuan Yang (2007) Amino Acid-coded Tagging-based Approaches in Quantitative Proteomics. Expert Review of Proteomics, 4:25-37.
  • Tianyi Wang, Tapani Ronni, Sheng Gu, Susan Sun, Helen Yin, and Xian Chen* (2006) Fliih Negatively Modulates the MyD88-dependent Signaling Pathway. Journal of Immunology 176:1355-1362.
  • Yu-Chun Du, Sheng Gu, Jian,hong Zhou, Tianyi Wang, Hong Cai, Mark A. MacInnes, E. Morton Bradbury, and Xian Chen* (2006) The Dynamic Alterations of H2AX Complex during DNA Repair Detected by a Proteomic Approach Reveal the Critical Roles of Ca2+/Calmodulin in the Ionizing Radiation-induced Cell Cycle Arrest. Mol. Cell. Proteomics, 5:1033-1044.
  • Sheng Gu, Zhihe Liu, Songqin Pan, Zeyu Jiang, Huimei Lu, Or Amit,, Jian Yu, Chien-An A. Hu, Xian Chen* (2004) Global Investigation of p53-Induced Apoptosis Through Quantitative Profiling Regulatory Proteins Using Comparative Amino Acid-Coded Tagging Proteomics. Mol. Cell. Proteomics. 3(10)998-1008.

Lab Contact:

Lab Rooms: 3072 Genetic Medicine
Lab Phone: 919-966-7489
Fax: 919-966-2852
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