The UNC CF Center has an extensive array of technologies to measure ciliary function both in vitro and in vivo. Thus, the Center has available techniques for measurement of ciliary beat frequency in human and animal culture systems, measurements with high-speed videomicroscopy of ciliary beat form, ultrastructural measurement capacities to evaluate ciliary shaft structure, and the ability to perform proteomics of ciliary shaft proteins (see Figure 12, and Figures 13-14).

Figure 12. Fractionation of ciliary proteins by SDS-PAGE. Each band was subjected to in-gel digestion and analyses by LC/MS^E to identify and quantify the entire ciliary proteome.