Mackman Lab Publishes Two Articles
https://www.rpthjournal.org/article/S2475-0379(25)00331-0/fulltext
https://www.rpthjournal.org/article/S2475-0379(25)00340-1/fulltext
“Optimization of a Western blot protocol for the detection of low levels of tissue factor in human cells”
The tissue factor (TF)/activated factor VII complex is the major activator of the coagulation system. TF is expressed by a variety of cells, including activated monocytes and tumor cells. Increased TF expression can cause thrombosis in different diseases, including sepsis, viral infections, and cancer. We have previously described a method for analyzing human TF in high-expressing cells by Western blotting.
and
“Evaluation of a new commercial assay for the measurement of tissue factor activity of extracellular vesicles isolated from human plasma”
Tissue factor (TF)-positive extracellular vesicles (EVs) are released into the circulation and activate coagulation in several diseases, such as sepsis, viral infections, and cancer.