Histology – Sample Images
Basic Histological Stains
DAB and Fluorescent IHC |
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| CME stain (mouse artery) Elastic Fibers—black, Collagen, mucin—green Muscle—red/brown |
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| Masson Trichrome stain (Mouse heart valves) Nuclei—black Cytoplasm, muscle fibers—red Collagen, mucin—blue |
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| Periodic Acid-Schiff stain (PAS) Glycogen—bright rose/red Nuclei—blue
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| Prussian Blue stain for iron Nuclei, hemofuschin—bright red Hemosiderin(iron)—blue Background—pink |
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| Sudan IV stain– lipids Fat—red Nuclei—blue |
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Von Kossa stain- Calcium deposits
Calcium—black Nuclei, muscle—red |
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| BrdU staining |
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| Biocytin IHC with DAB visualization- neuron |
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| Lysozyme IHC with DAB visualization |
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| Fluorescent double-label with GFP and Biocytin-SATR (neuron)
Green- GFP Red- Biocytin-SATR Yellow- Co-expression |