Basic Histological Stains

DAB and Fluorescent IHC

histo6
CME stain (mouse artery)
Elastic Fibers—black,
Collagen, mucin—green
Muscle—red/brown
histo8
Masson Trichrome stain (Mouse heart valves)
Nuclei—black
Cytoplasm, muscle fibers—red
Collagen, mucin—blue
histo1
Periodic Acid-Schiff stain (PAS)
Glycogen—bright rose/red
Nuclei—blue

histo7
Picrosirius Red stain (gut)
Collagen—red

histo2
Prussian Blue stain for iron
Nuclei, hemofuschin—bright red
Hemosiderin(iron)—blue
Background—pink
histo4
Sudan IV stain– lipids
Fat—red
Nuclei—blue
histo3
Von Kossa stain- Calcium deposits
Calcium—black
Nuclei, muscle—red
histo9
BrdU staining

histo5
Biocytin IHC with DAB visualization- neuron
histo10
Lysozyme IHC with DAB visualization
histo11
Fluorescent double-label with GFP and Biocytin-SATR (neuron)

Green- GFP

Red- Biocytin-SATR

Yellow- Co-expression