Services Provided
Service Provided
Training in All Aspects of CryoEM
- Theory and principles of cryoEM
- Specimen preparation using Vitrobot Mark IV cryo-plunger
- Operation of the 200 keV Talos Arctica CryoTEM
- Assistance in 3D image analysis
Specimen Preparation
- Preparation of cryo grids by rapid immersion in liquid ethane using FEI Vitrobot Mark IV
- Fabrication of thin films for CryoEM application including graphene, thin carbon and lipid affinity grids.
- Long-term storage of cryogrids
CryoTEM of Nano-particles
- Automated low-dose image, montaging, and data collection
- Data analysis and annotation
Single-Particle CryoEM
- Screening of cryo grids to assess sample quality
- High-resolution single-particle cryoEM data collection
- On-the-Fly data processing
- Data processing
Cryo-Electron Tomography
- Automated cryoET data collection
- On-the-Fly data processing using WARP
- Data processing
Core Rates
Vitrobot
- Internal $90 per hour
- External $182 per hour
- Industry $200 per hour
Talos Arctica
- Internal $1,500 per day
- External $2,490 per day
- Industry $3,000 per hour
Sputter Coater
- Interal $99
- External $181
- Industry $200
Labor
- Internal $99 per hour
- External $154 per hour
- Industry $160 per hour
Consultation
- Internal $190
- External $427
- Industry $430
Cryo Specimen Storage
- Internal $445
- External $693
- Industry $800
Clipping Station
- Internal $36
- External $67
- Industry $67
Grid Fabrication
- Internal $710
- External $1,105
- Industry $1,200
Dry Shipper
- Internal $168
- External $262
- Industry $350
For all inquiries please contact Dr. Joshua Strauss mailto:Joshua_Strauss@med.unc.edu