Welcome to the Cryo Electron Microscopy (CryoEM) Core at UNC Chapel Hill

We provide services and training in all aspects of cryoEM sample preparation, screening and high-resolution data collection. Check out our Services and don’t hesitate to Contact Us if you have any questions!

Check out some of our high-resolution structures!

1.77Å Apoferritin, processed by Jared Peck and Jon Fay

1.96Å Glutamine Synthetase, processed by Jared Peck

2.4Å Nucleosome Core Particle, processed by Jared Peck

3.3Å cGAS-Nucleosome complex, processed by Cathy Spangler

2.5Å GPCR complex, processed by Jon Fay

Apoferritin 2.47 Å, from 25 tilted micrographs collected at 45 degrees — Joshua Strauss

AP2 bound to heparin in the closed conformation 3.4 Å (EMD-24710), processed by Dr. Rick Baker.

Lipoprotein Lipase Dimer 3.9 Å (EMD-28554), processed by Dr. Kathryn Gunn. Collected at 0, 30 and 45 degrees tilted.

Organic cation transporter 1 in complex with verapamil 3.45 Å (EMD-28588), processed by Dr. Yang Suo.

In addition to high-resolution single-particle cryoEM, we also provide cryoTEM analysis of nanoparticles and cryo-electron tomography as a fee-for-service.

CryoTEM Micrograph of Lipid Nanoparticles

Sample provided by Dr. Menglin Wang and Dr. Huang Leaf.

Scale bar 50 nm

CryoET of Lipid Nanoparticles

Courtesy of Dr. Menglin Wang and Dr. Huang Leaf.

We are committed to providing researchers at UNC-Chapel Hill with the resources, training, and technical assistance required to conduct high-resolution cryoEM experiments.

We obtain ongoing support from the UNC Lineberger Comprehensive Cancer Center through the University Cancer Research Fund and the Cancer Center Support Grant. Consequently, publications supported by the UNC Center for Structural Biology must acknowledge NIH grant P30CA016086 and be submitted to PubMed Central in compliance with the NIH Public Access Policy.

Suggested acknowledgment: “This work was supported by the National Cancer Institute of the National Institutes of Health under award number P30CA016086. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.”

Featured image at top of this page: This is a close-up photo of the centerpiece of the CryoEM Core Facility: a 200 Kev Thermo Fisher Scientific Talos Arctica G3 CryoTEM and Gatan K3 direct electron detector.

“This is real science…it just looks like science fiction.” ~ B. Clarke