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Educational Video Lectures

Caltech Cryo-EM-Course by Dr. Grant Jenson http://cryo-em-course.caltech.edu/videos

National Resource for Automated Molecular Microscopy (NRAMM) and the Simons Electron Microscopy Center (SEMC) at the New York Structural Biology Center videos https://www.youtube.com/channel/UCgd1dW4HQ_yvpRjFc670htw/featured 

MRC Molecular Biology Laboratory CryoEM course  https://www2.mrc-lmb.cam.ac.uk/research/scientific-training/electron-microscopy/

Online Training and Learning

MYSCOPE Microscopy Training

An online learning environment for electron microscopy and other imaging techniques.  Covers the theory, instrument design, applications, and practicals to test your knowledge.  Highly recommend it for people interested in learning TEM.

https://myscope.training

Electron Microscopy Terms

Comprehensive list of microscopy terms from JEOL Ltd.  This site contains a glossary of 800 terms, with illustrations and technical information.  New terms are constantly being added or updated.  A great resource for learning and for teaching cryoEM.

https://www.jeol.co.jp/en/words/emterms/

CryoEM 101

An online cryoEM learning environment in the format of a textbook.  Covers the basis of sample purification, cryo-grid preparation, screening, data collection, and image processing.  A great resource for people interested in getting started in single-particle cryoEM.

https://cryoem101.org/chapter-1/#

Software for CryoEM image processing

RELION https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page

CisTEM https://cistem.org

CryoSPARC https://cryosparc.com

EMAN2 https://blake.bcm.edu/emanwiki/EMAN2

IMOD https://bio3d.colorado.edu/imod/

SPIDER https://spider.wadsworth.org/spider_doc/spider/docs/spider.html

Scipion http://scipion.i2pc.es

How to process your data without a GPU Linux Workstation

Process data on a virtual machine http://gcpexamplesforresearch.web.unc.edu/2019/01/cryo-em-workstation/

Process data on the Dogwood computer cluster https://its.unc.edu/research-computing/dogwood-cluster/

Turn the Key Linux Workstations for CryoEM Application

Single Particle LLC https://www.singleparticle.com 

Exxactcorp https://www.exxactcorp.com/Relion-for-Cryo-EM-Solutions

TEM Grids for CryoEM

We typically use TEM grids with hole size and spacing of R1.2/1.3 or R0.6/1.  We prefer to use copper or gold 300 mesh TEM grids but 200, and 400 mesh will also work fine for single-particle cryoEM.  TEM grids with regular spaced holes can be purchased from vendors like EMS or Tedpella or direct from the manufacturers Quantifoil or Protochips.

Quantifoil https://www.quantifoil.com/

Protochip https://www.protochips.com/

Software for data acquisition.

To collect data on the UNC Talos Arctica we use the software package SerialEM.

SerialEM http://bio3d.colorado.edu/SerialEM/

UNC CryoEM Core SerialEM Tutorial from the UNC CryoEM Journal Club

20200506_SerialEM_JDS

Protocol for screening and collecting data with the UNC Talos Arctica

20200811_UNC_Talos_Arctica