CryoEM Links and Resources
Educational Video Lectures
Caltech Cryo-EM-Course by Dr. Grant Jenson http://cryo-em-course.caltech.edu/videos
National Resource for Automated Molecular Microscopy (NRAMM) and the Simons Electron Microscopy Center (SEMC) at the New York Structural Biology Center videos https://www.youtube.com/channel/UCgd1dW4HQ_yvpRjFc670htw/featured
MRC Molecular Biology Laboratory CryoEM course https://www2.mrc-lmb.cam.ac.uk/research/scientific-training/electron-microscopy/
Online Training and Learning
MYSCOPE Microscopy Training
An online learning environment for electron microscopy and other imaging techniques. Covers the theory, instrument design, applications, and practicals to test your knowledge. Highly recommend it for people interested in learning TEM.
Electron Microscopy Terms
Comprehensive list of microscopy terms from JEOL Ltd. This site contains a glossary of 800 terms, with illustrations and technical information. New terms are constantly being added or updated. A great resource for learning and for teaching cryoEM.
An online cryoEM learning environment in the format of a textbook. Covers the basis of sample purification, cryo-grid preparation, screening, data collection, and image processing. A great resource for people interested in getting started in single-particle cryoEM.
Software for CryoEM image processing
How to process your data without a GPU Linux Workstation
Process data on a virtual machine http://gcpexamplesforresearch.web.unc.edu/2019/01/cryo-em-workstation/
Process data on the Dogwood computer cluster https://its.unc.edu/research-computing/dogwood-cluster/
Turn the Key Linux Workstations for CryoEM Application
Single Particle LLC https://www.singleparticle.com
TEM Grids for CryoEM
We typically use TEM grids with hole size and spacing of R1.2/1.3 or R0.6/1. We prefer to use copper or gold 300 mesh TEM grids but 200, and 400 mesh will also work fine for single-particle cryoEM. TEM grids with regular spaced holes can be purchased from vendors like EMS or Tedpella or direct from the manufacturers Quantifoil or Protochips.
Software for data acquisition.
To collect data on the UNC Talos Arctica we use the software package SerialEM.