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Attune NxT

 

The ThermoFisher Attune NxT is equipped with a 96/384 well plate Autosampler, 4 lasers (405, 488, 561, and 637nm) and can detect up to 14 fluorescent parameters. Its acquisition software is Attune NxT 7.1.0.

Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on both Attune 1 (B018) and Attune 2 (B021A).

The FlowCore has two Attune NxT instruments. The Optical layouts remain the same between both instruments, though the voltage settings may be different. View the optical laouts with Core-generated starting voltages below:

Attune V6 configuration (Attune 1 Voltages) Rev. 9-25-2025

Attune V6 configuration (Attune 2 Voltages) Rev. 9-25-2025

For Panel Design help, Contact the Flow Core and/or use EasyPanel.

The Attune uses acoustic focusing to be able to acquire samples at high speeds (12.5-1000 uL/min). To ensure the best flow, concentrate your cells appropiately: Attune NxT cell concentrations

UT-SOP007.5-Thermo-Fisher-Attune-NxT-User-Training. Last Updated 01/07/2026.

The instrument can take tubes (1.5 mL or 5 mL) or plates. See Validated Plates here: Validated Plates