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Asked to submit a form? Need further information for services we provide? HTSF wants to ensure that you have a central location to find all forms and supplemental information for all services we provide. If there is additional information needed, not listed here, please contact our customer service team.

General

Requesting a Quote

This form is for potential or current customers seeking accurate pricing for an upcoming project being planned and needing exact/precise numbers for grant submission, etc. This may be filled out online OR down loaded to be filled out and emailed the HTSF customer service group.

Fill out Requesting a Quote Form online

HTSF DNA and RNA Sample Preparation Requirement Guide

This is a quick guide to properly preparing RNA and DNA samples for submission. Listed by raw material type, this table lists minimum concentrations, volume and quality metrics required for different library types.

It also indicates for a limited set of library types, what to submit if 2 libraries are to be made from 1 submitted aliquot.

  • Total RNA and Small RNA
  • mRNA and Small RNA

If you have any further questions on submitting material, please contact or lab manager, Chris Nagy at cnagy@email.unc.edu

HTSF Shipping & Packing Instructions

Information on shipping and packing samples to HTSF

Sequencing Platform Timetable

This table will help customers understand how long it will take to see their data in their project folder after receiving a “SEQUENCING STATUS” email. It includes average run time to data delivery.

FAQ’s

This is a list of our most frequently asked questions. They range from submission information to QAQC questions to the correct HTSF staff member to contact about specific questions. If you have a question(s) that are not listed, continue to reach out to the customer service team

Where is the HTSF?

This is for our new customers who are unsure of where we are located. Use this guide for directions from multiple locations on campus

HTSF Capabilities

This is a list of all the technologies we offer at HTSF. We have a wide variety of capabilities and are always looking for new and improved sciences.

HTSF Policies

This guide will help understand how HTSF operates and who to contact if you have questions referring to billing, data delivery, etc.

Tracseq and Submissions

TracSeq Step by Step Submission Guide

This is an introduction to working with TracSeq, step-by-step guide for submitting samples, and TracSeq site features

A YouTube video on the 7 step submission process

How to Fill Out a TracSeq Manifest Help Guide

This includes key points for filling out a manifest necessary for sample submission

HTSF-Drop-Off Checklist Guide

Summary checklist for submitting and dropping off samples at the HTSF and includes COVID non-contact drop off instructions.

Submitting Barcodes to HTSF

This guide will help you submit your single and dual barcodes correctly to your sample manifest on TracSeq

Submitting Custom Sequencing Primers to HTSF

This guide specifies the requirements needed for submitting custom primers for your material

HTSF Approved Container Requirements

This is a guide to help decide which container to use when submitting material to HTSF. This the most updated list of samples we DO accept, along with pictures and names of samples we DO NOT accept

External Project Sample Submission Form

The TracSeq submission site requires UNC authenticated user permission. The Customer service group is required to submit for external users. This form is to be filled out and sent to the Customer Service group for submission.

You can also fill out an External Project Sample Submission Form online.

Navigating TracSeq

This a step by step guide for using TracSeq beyond submissions. How to find:

  • Account names and batch numbers
  • Viewing quotes
  • Printing out confirmed manifest for drop off
  • Finding attachments for : sample QAQC, Submission, etc
  • Flowcell information: loading info, run names, run metrics
  • Data delivery folder

Illumina

Account Initiation Document

This form is the first step for anyone planning to utilize Illumina technology. It provides information about the samples being submitted for sequencing and analysis, as well as the larger context of the study. Please send completed form to the HTSF Customer Service Team.

Account Update Request Form

This form is used when there has been a significant amount of time since the last submission from a study or new requirements need to be added to a study.

Illumina Sequencing Platform Comparison Chart

This is a chart to help guide customers with picking sequencing platforms. It includes all of our Illumina sequencing options and is a great tool to compare MiSeq, older HiSeq platforms and NovaSeq6000.

This is a guide for those who are not completely familiar with NovaSeq6000 technology and are curious about the amount of data the NovaSeq is able to produce per flowcell vs lane.

NovaSeq Requirements and Pooling Suggestions

This table lists the requirements for a pool to be run on the various NovaSeq Platforms. It also has a table for the suggested number of libraries per pool based on library type and NovaSeq versions.

PhiX Addition

A brief discussion from Illumina on the need for PhiX and when to increase the percentage used.

FAST-SEQ Release Form

FAST-SEQ sample expectations and release form. Prior to submitting for FAST-SEQ. A consult with Tara Skelly and Amy Perou is required

Alternative Technologies

HTSF Exosome Final Prep during Isolation

How to submit for 10x Protocol for the final isolation steps for exosomes. Exosomes cannot be frozen down in a typical manner for HTG library prep to work. They need to be frozen down in the buffer used for lib prep. See Protocol for details.

10x Genomics Submission Instructions for STUDY MADE libraries

How to submit for 10x libraries that were made by the study.

  • Planning of the experiments including discussion of cell numbers, pooling and sequencing
  • Filling out a TracSeq request
  • Used in conjunction with 10x Genomics Manifest for Study Made Libraries with Indexes and Sequencing Requirements

10x Genomics Manifest for Study Made Libraries with Indexes and Sequencing Requirements

When preparing 10x libraries that will be submitted to the HTSF, additional requirements and information are needed to ensure samples are processed correctly on Cell Ranger. This manifest includes:

  • Additional tabs – barcode nt seq for each of the barcodes in the index sets.
  • Sequencing cycle set up for the different library kits based on version number and Seq platform.

10x Genomics Submission Instructions for HTSF libraries

How to submit for the HTSF to make 10x libraries.

  • Planning of the experiments including discussion of cell numbers, pooling and
  • Filling out a TracSeq request
  • Used in conjunction with 10x Genomics Library Indexes and Sequencing Requirements
  • Confirmation of sample delivery to the HTSF

10x Genomics Library Indexes and Sequencing Requirements

This is a quick guide to help properly prepare 10x samples. This guide has tabs for:

  • Barcode nt seq for each of the barcodes in the index sets.
  • Sequencing cycle set up for the different library kits based on version number and Seq platform

10x Genomics Submission Manifest for HTSF Made Libraries

This is an HTSF internal Document for pooling of 10x libraries. This should be attached to the submission batch for the bioinformatics group to use for cell ranger demux.

Long Read Technologies

Oxford Nanopore Submission Form

This form should be filled and returned to Tara Skelly and Piotr Mieczkowski prior to sample drop off

BioNano Submission Instructions

This is a quick guide to properly prepare for BioNano submissions. Please note, a consult with Piotr Mieczkowski and Ewa Malc is required prior to sample drop off

Bionano Submission Form

This form should be filled and returned to Ewa Malc and Piotr Mieczkowski to ensure project setup and sample processing timeline

White Papers

White papers are a general synopsis of each described technology. If you need further information on any of these topics, check our Technologies Tab on the HTSF website OR please feel free to reach out to the HTSF customer service team.

NovaSeq

Discusses Illumina NovaSeq Platform Technology and considerations.

Oxford Nanopore

Discusses Oxford Nanopore Technology and considerations.

Bionano

Discusses Bionano Saphyr Technology and considerations.

10x Genomics

Discusses 10x Genomics Technology, suggested cell inputs and considerations.

HTG Edge

Discusses HTG Technology and considerations.

Grant Writing

This set of forms and guides are to help HTSF customers write grants and use HTSF if official publication along with details of who to contact if needing further assistance with this type of work

Requesting Grant Letter of Support

Fill out this form and return to the HTSF customer service group when you would like a letter of support from our directors.

Grants and Papers Guide

A variety of information that can be helpful when writing. Including how to acknowledge the HTSF in publications.

HTSF Rigor and Reproducibility

This is an extension of our policies that specifies our beliefs and standards to continue progression at HTSF