DNA Library Preparation and Sequencing
Complete Genome Re-Sequencing

Library preparation recommendations include:
- Kapa Hyper DNA seq
- Celero, if requesting PCR free and submitting at least 2 micrograms of DNA
Sequencing platform and pooling recommendations can be found below:
Services | Samples per Pool | Platform | Cycles |
DNAseq Human Genomes | 24 | NovaSeq S4 | 2×150 |
If there are substantial gaps in the sequence assembly, other platforms such as Oxford NanoPore GridIon or Pacific Biosciences Sequel can frequently be used to create larger contiguous assemblies to supplement the deep coverage from Illumina sequence runs. More information on these platforms can be found under the Technologies tab, Long Read Technologies.
Low-Input Double Stranded DNA Products

Library preparation recommendations include:
- Thruplex kits with low-input material
- Kapa Hyper DNA seq
Sequencing platform and pooling recommendations can be found below:
Services | Samples per Pool | Platform | Cycles |
|
125 | NovaSeq S4 | 2×50 |
48 | NovaSeq S2 | ||
20 | NovaSeq S1 | ||
10 | NovaSeq SP |
**NOTE: Although the NovaSeq is recommended for this service, the HiSeq 4000 is also an available platform. A maximum of 3-4 libraries per lane on the HiSeq 4000 is recommended.
Custom Library Preparation and Sequencing


Do not see something you are interested in? Contact us we are always willing to try new novel library preparations through our R&D group.
Reduced Representation Bisulfite Sequencing (RRBS)

Services | Samples per Pool | Platform | Cycles | |
RRBS | 200 | NovaSeq S4 |
2×100 |
|
74 | NovaSeq S2 | |||
32 | NovaSeq S1 | |||
16 | NovaSeq SP | 2×150 | ||
6 | HiSeq 4000 | 2×100 |
Metagenomic sequencing

Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. In medical research discovery of infectious and commensal flora relies increasingly on metagenomics analyses.
Library preparation options for this service include:
- 16S for bacterial samples
- 18S or ITS for Eukaryote samples
Sequencing platform and pooling recommendations can be found below:
Services | Samples per Pool | Platform | Cycles |
Metagenomics DNAseq | 15 | HiSeq 4000 | 2×150 |
40 | NovaSeq SP | ||
80 | NovaSeq S1 | ||
190 | NovaSeq S2 | ||
500 | NovaSeq S4 | ||
16S | 96 | MiSeq | 2×300 |
Restriction Site Associated DNA Sequencing (RAD)
Restriction site associated DNA (RAD) markers are a type of genetic marker which are useful for association mapping, QTL-mapping, population genetics, ecological genetics and evolution genetics. An important aspect of RAD markers and mapping is the process of isolating RAD tags, which are the DNA sequences that immediately flank each instance of a particular restriction site of a restriction enzyme throughout the genome.Once RAD tags have been isolated, they can be used to identify and genotype DNA sequence polymorphisms mainly in form of single nucleotide polymorphisms (SNPs). Polymorphisms that are identified and genotyped by isolating and analyzing RAD tags are referred to as RAD markers.
UNC has a collaboration with NCSU’s Genomic Sciences Laboratory (GSL) to offer RAD-Seq at the same internal cost you would receive if you were sequencing on UNC’s Campus. GSL website:
For project quotes, questions, and consults:
Contact Dr. David (Andy) Baltzegar, GSL Director
Phone: 919.513.0738 (8a – 4:30p Mon- Fri)
Email: dabaltze@ncsu.edu
Relevant DNA Services Forms and Guides
HTSF Sample Preparation Requirement GuideIllumina Platform Comparison and Specification Table
NovaSeq Requirements
NovaSeq White Paper