Alternative Library Services

HTG Sequencing

HTSF no longer offers this service. HTSF now collaborate with Dr. Deepak Kumar’s lab at NCCU. Dr Kumar’s group makes the different libraries and then sends them to UNC HTSF for sequencing to get the required depth of data.

Please contact:

Lesli Rios-Colon (lrioscolon@nccu.edu)

Elana Arthur (earthur1@nccu.edu)

HTG Molecular

HTG Molecular’s EdgeSeq is a fully automated sample and library preparation platform for targeted RNA sequencing that pairs HTG’s extraction-free, high-specificity Edge Chemistry with the high sensitivity and dynamic range of next-gen sequencing. EdgeSeq enables digital quantitation of miRNA and mRNA expression from difficult sample types such as formalin-fixed, paraffin-embedded (FFPE) tissues, plasma and exosomes.

Assays that are available with HTG include:

• HTG EdgeSeq Oncology Biomarker Panel (OBP)
• HTG EdgeSeq Precision Immuno-Oncology Assay (IO)
• HTG EdgeSeq miRNA Whole Transcriptome Assay (miRNA)
• HTG EdgeSeq PATH Panel (PATH)
• HTG EdgeSeq Lymphoma Panel (DLBCL)
• HTG EdgeSeq Mouse mRNA Tumor Response Panel (TRP)

Sequencing and pooling recommendations can be found in the chart below:

For more information on HTG, please visit https://www.htgmolecular.com/

10X Genomics

Single Cell Gene Expression (sc 10X)

The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. It can help to uncover gene expression variability and identify rare cell types in heterogeneous samples to better characterize cellular contributions during development and disease.

Optionally with Feature Barcoding Technology
10X Genomics’s latest improvements allow researchers to:

1. CRISPR Screening – identify cell-specific CRISPR-mediated perturbations on gene expression via direct capture of gRNAs and polyadenylated mRNAs from the same single cell
2. Cell Surface Protein – measure both gene and cell surface protein expression in the same cell to identify protein isoforms, detect protein for low abundance transcripts, and further increase phenotypic specificity

Single Cell ATAC

Single Cell ATAC accelerates the understanding of the regulatory landscape of the genome, thereby providing insights into cell variability. The chromatin profiling of tens of thousands of single cells in parallel allows researchers to see how chromatin compaction and DNA-binding proteins regulate gene expression at high resolution.

Sequencing and pooling recommendations can be found in the table below, A more complete lost of cycles and platforms can be found on 10x Genomics Library Indexes and Sequencing Requirements in Forms and Guides, Alternative Technologies.

Sequencing and pooling recommendations can be found in the table below:

HTSF is unable to offer 10X Genomics library prep at this time. Please contact the Advanced Analytics Core (AACORE). There is detailed AACORE info and contact listed on this website under the About Us section, UNC HTSF Collaborating Cores page. AACORE offers complete single cell services for 10 Genomics library prep. When deeper sequencing is required, AACORE transfers the 10x pools to the HTSF for sequencing.

For more information on 10X genomics, please visit https://www.10xgenomics.com/

Relevant Alternative Services Forms and Guides