Library Preparation
The HTSF offers multiple library preparation services that include:
- Illumina based technology libraries – manual and robotics production
- Non-Illumina based library preparation
- Custom libraries
- R&D novel library preps – with consultation with HTSF
- Oxford Nanopore and Bionano Libraries
Do not see a library you are thinking about? Have an idea but not sure how to go about it? Come speak with HTSF about your needs, we can make suggestions as to the best options to fit your budget and data needs.
Illumina Based Library Preps
A selection of standard library preparation services that are currently offered by the HTSF can be found in the below chart. Our Illumina based preparation have Illumina adapters and can therefore sequence on Illumina machines such as the NovaSeq. Many of these are high throughput and made using our robotics. HTSF has a lengthy list of non-standard libraries which are often manual production in smaller sample number sets. For more information on HTSF services, including pooling and platform recommendations for these library preps, please visit our Services tab (RNA Library Preparation and Sequencing and DNA Library Preparation and Sequencing).
Standard Illumina-based Library Preparation
| Submitted Material | Library Type | Library Procedure |
| Amplicon
|
Amplicon
|
Kapa Hyper |
| Nextera XT prep | ||
| Thruplex DNA-seq | ||
| ChIP DNA | ChIP Seq | Thruplex DNA-seq |
| FAIRE DNA | FAIRE Seq | |
| Total RNA | mRNA | Kapa Stranded mRNA |
| NUGEN mRNA stranded Libraries (from Blood Source) | ||
| Total RNA | Kapa RNA – Ribo Erase | |
| TruSeq Stranded RNA Ribo-Zero Gold (Human/Mouse/Rat) | ||
| NUGEN_ Ovation Total RNA depletion non stranded libraries (drosophila, blood, plant, microbe, or fetal blood) | ||
| NUGEN Ovation SOLO RNA seq (Human) | ||
| NUGEN Ovation SOLO RNA seq (Mouse) | ||
| NUGEN Ovation TRIO RNA Seq | ||
| Small RNA (miRNA) | BioO Scientific NEXT Flex-v3 | |
| Total RNA (<100ng) | mRNA | SMARTer Ultra Low Input RNA + Nextera XT RNA |
| Total RNA | TruSeq RNA Access | |
| Whole Genomic DNA | 16s Metagenomics | 16s Metagenomics — HTSF primer set: V2 or V3 or V4 molecule tags |
| 16S and ITS | 16s Metagenomics and Internal Transcribed Spacer (ITS) libraries
|
|
| DNA Seq
|
||
| Kapa Hyper | ||
| Kapa Hyper on the Mantis at 1/3 reagent | ||
| Nextera XT prep | ||
| Nextera Rapid Capture Exome | ||
| Nextera Flex | ||
| NUGEN Celero |
Alternative Library Preparation Services
In addition to Illumina library preparation services, the HTSF works with other core for non-Illumina based libraries. A selection can be found in the below chart. More information on these services can be found in the Alternative Library Technologies subsection of the Technologies tab.
Alternative Library Preparation Services
| Submitted Material | Library Type | Library Procedure |
|
HTG EdgeSeq | Library prep + Capture in the following assays (HTG is developing more uses):
|
| Single Cell | 10X Chromium Genomics: Single Cell | Single Cell 3’ |
| 10X Chromium Genomics: ATAC | Single Cell ATAC Seq |
Custom Preparations
The HTSF offers custom library preparations upon consult with HTSF management. We are happy to work with you on novel ideas for NGS and beyond. Please contact our customer service team to further discuss your custom library preparation needs or if you are interested in a new library preparation method that is not listed on our website. HTSF is constantly exploring and testing new library preps that come on the market. We will set up a zoom with the appropriate HTSF members.Currently offered custom preparation services include:
Custom Library Preparation Services
| Submitted Material | Library Type | Library Procedure |
| Total RNA | Total RNAseq | Total RNA with ribosomal depletion of non-Human/Mouse/Rat models |
| Whole Genomic DNA | Custom Capture | Nextera Rapid Capture Exome |
| KAPA Hyper DNA Prep using Lockdown probes from IDT (includes Capture + Standard HTSF probe set for whole Human Exome, 36 mega base Target) | ||
| RRBS | Reduced Representation Bisulfite Sequencing | |
| DNA Methyl Seq | MethylSeq / Epignome | |
| DNA seq | SureSelect V10 |
Oxford Nanopore and Bionano Library Preparation Services
The HTSF also offers both Oxford Nanopore and Bionano based libraries preparation services. Please contact the HTSF for information. And see more info on these service in the Technologies tab, Long Read Technologies.Relevant Library Services Forms and Guides
HTSF Sample Preparation Requirement GuideApproved Containers Information