The MMRRC at UNC will use cryopreserved sperm to perform an IVF using female donors (C57BL/6, SW or FVB). Other backgrounds will incur a higher fee due to the increased cost of females and quantity needed for a successful IVF. The resulting embryos are transferred to 1 psuedo-pregnant female.
What we need
- At least 2 aliquots of cryopreserved sperm. Aliquots must be shipped directly to the MMRRC at UNC to ensure the proper handling.
- Thaw protocol
- Genetic background
- QC information for that sample
What you get
- MMRRC at UNC will perform an IVF with the cryopreserved sperm within 1 month of receiving the sperm. This is due to the time needed to order donor females.
- Resulting embryos will be transferred to 1 pseudo-pregnant female.
- Vendor quality mice (restricted flora)
- Health report for your mice.
- If genotyping confirmation is required, we will take tail snips when mice are 10 days old. Frozen tail snips will be available for pick up by the investigator’s lab.
- If the procedure is successful, mice will be transferred to your colony in about 16 weeks after the IVF.
Pricing (1 day of IVF)
|Sperm Cryorecovery with common backgrounds (C57BL/6, SW and FVB)||$2275|
|Sperm Cryorecovery with uncommon backgrounds||Please email for quote.|
Sperm must be shipped to the MMRRC at UNC directly to ensure proper handling of the aliquots. The MMRRC at UNC cannot make any guarantees that this procedure will be successful. Since the MMRRC at UNC did not cryopreserve the sperm, we cannot guarantee the quality of the sample. It is recommended to submit multiple aliquots of sperm for reanimation. We will attempt an IVF with 1 aliquot and save the others for back up. The thaw protocol must be submitted when the sperm aliquots are received. Without the proper thaw protocol, the MMRRC at UNC cannot perform an IVF.
If the sperm has low fertilization and doesn’t provide enough embryos for a transfer, we can attempt the IVF again for a reduced fee ($1675).
If pups are born, the surrogate will be health tested after pups are weaned. Due to the strict health standards of our colony, every surrogate that births must be sent to Charles River Laboratory for full health testing. The cost of health testing is included. It will take approximately 16 weeks until mice are ready to be transferred to your colony. If genotype verification is needed, we can tail snip the resulting pups at 10 days old. The tail snips will then be available for pick up by the investigator’s lab.