Equipment
ThermoFisher Scientific Talos Arctica CryoTEM
200 keV transmission electron microscope.
FEG-X Gun Schottkey Emitter.
Constant power C-Twin object lens with Cs = 2.7 mm.
Autoloader capable of holding 12 cartridges.
Ceta-D 4096 x 4096 pixel CMSO CCD.
Gatan K3 5,760 x 4,092 pixel direct electron detector.
Automated single-particle, electron tomography, and micro-election diffraction data collection using SerialEM or EPU-D.
On-the-fly processing using cryoSPARC Live.
Capable of obtaining near atomic resolution for single-particle cryoEM. Benchmarked with Apoferritin sample 1.8 Å resolution based on FSC=1.34.
Thermo Fisher Scientific Vitrobot Mark IV
Robotic cryo-plunger capable of semi-automated vitrification.
Capable of manual or automated blotting.
Up to 16 grids can be prepared per freezing session.
Touch screen consul and foot-pedal for hands-free operation.
Enclosed processing chamber that maintains high humidity (100%) and temperature (4–60°C) for samples.
Precise control of vitrification parameters including blot time.
We use a mixture of ethane (40%) propane (60%) as the primary cryogen. This much easier to use as compared to pure ethane.
Pelco easiGlow
Glow discharge cleaning system for rendering TEMs hydrophilic for CryoEM application.
Easy to use and automated.
Unit uses air as source of plasma.
Easy to use touch panel with predefined settings.
Adjustable stage glass slide holder can hold multiple grids.
Pie Scientific Tergeo-EM plasma cleaner
Oil-free dry pump.
Three gas delivery ports with precise mixing capabilities. Gases available for general use include argon, oxygen and hydrogen.
Custom recipes for CryoEM applications for different types of TEM grids including (1) Quantifoil, (2) Lacey Carbon, (3) TEM grids with continuous layer of thin 2-nm carbon, (4) C-Flat, (5) UltrAufoil TEM grids.
Leica EM ACE600 Sputter Coater
High vacuum coating system for carbon and sputter coating.
Used to prepare ultra thin (2-4 nm) carbon for single-particle CryoEM applications.
Used to prepare UltrAufoil style gold grids for single particle CryoEM application.
Graphite rods and gold sputter target available for general use.