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Project planning and consultation for CryoEM

Training in all aspects of CryoEM

  • Theory and principles of cryoEM
  • Specimen preparation using Vitrobot Mark IV cryo-plunger
  • Operation of the Talos Arctica CryoTEM
  • Assistance in 3D image analysis

Specimen preparation

  • Preparation of cryo grids by rapid immersion in liquid ethane using FEI Vitrobot Mark IV

CryoTEM

  • Screening of cryo grids to assess sample quality
  • High-resolution single-particle cryoEM data collection
  • Cryo-electron tomography
  • Data pre-processing and on-the-fly analysis

For all inquires please contact Dr. Joshua Strauss mailto:Joshua_Strauss@med.unc.edu