Project planning and consultation for CryoEM

Training in all aspect of cryoEM

  • Theory and principles of cryoEM
  • Specimen preparation using Vitrobot Mark IV cryo-plunger
  • Operation of the Talos Arctica TEM
  • Assistance in image analysis

CryoEM specimen preparation

  • Preparation of cryo-grids by rapid immersion in liquid ethane using FEI Vitrobot Mark IV
  • Clipping and storing of cryogrids

Imaging of cryogrids

  • Screening of cryo-grids to assess sample quality and particle morphology
  • High-resolution single-particle cryoEM data collection
  • Preprocessing of images for single-particle cryoEM (motion correction and CTF correction)

For all-inquires please contact Dr. Joshua Strauss mailto:Joshua_Strauss@med.unc.edu