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Service Provided

Training in All Aspects of CryoEM

  • Theory and principles of cryoEM
  • Specimen preparation using Vitrobot Mark IV cryo-plunger
  • Operation of the 200 keV Talos Arctica CryoTEM
  • Assistance in 3D image analysis

Specimen Preparation

  • Preparation of cryo grids by rapid immersion in liquid ethane using FEI Vitrobot Mark IV
  • Fabrication of thin films for CryoEM application including graphene and thin carbon
  • Long-term storage of cryogrids

CryoTEM of Nano-particles

  • Automated low-dose image, montaging, and data collection
  • On-the-Fly data processing
  • Data analysis and annotation

Single-Particle CryoEM

  • Screening of cryo grids to assess sample quality
  • High-resolution single-particle cryoEM data collection
  • On-the-Fly data processing
  • Data processing

Cryo-Electron Tomography

  • Automated cryoET data collection
  • On-the-Fly data processing using WARP
  • Data processing

For all inquiries please contact Dr. Joshua Strauss mailto:Joshua_Strauss@med.unc.edu