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Lineberger Comprehensive Cancer Center

Core Access

The UNC Flow Cytometry Facility is open to all UNC-CH investigators. Additionally, we welcome investigators from outside institutions, including Duke University, NC State University, and companies within the Research Triangle Park (RTP). Access is managed on a first-come, first-served basis via iLabs.

 

Access to the Facility’s services requires an iLab account, linked to the laboratory’s Principal Investigator.

Steps to Register:

  1. Complete the iLab Account Sign-up Form.
    • UNC investigators: Select “University of North Carolina at Chapel Hill” from the dropdown menu rather than typing “UNC” to ensure correct recognition of the core.
  2. Once your account is approved, you will receive an email with account details and basic instructions to get started.

For assistance with managing iLab research groups or chartfield string entry, contact the SOM Business Office at coresupport@med.unc.edu

Technical Support

  • Click HELP at the top of the page to access the support portal and submit a ticket.
  • If unable to access the portal, email ilab-support@agilent.com or call 617-297-2805 with details of your issue.
  • You can also reach out to coresupport@med.unc.edu for iLabs help

Non-sorting, analysis-only flow cytometers are available for use in two ways:

  1. Facility Staff-Operated Use:
    • Facility staff will operate instruments for you. You must remain present at the instrument to consult as needed.
    • For recurring sample types, a “drop-off” service is available. Consult with staff if interested.
  2. User-Operated Use:
    • Investigators may operate instruments at a reduced cost, but training is required for each instrument type.
    • Training must be conducted by Facility staff solely and includes individual help sessions to ensure proficiency.
    • Note: Operating instruments is only recommended for users with substantial anticipated usage, as infrequent operation may lead to data quality issues.
    • Users may schedule their own instrument time unless Facility staff participation is required.

All sorting instruments are operated by Facility staff, with the exception of the FACSMelody, which is available for trained users to independently sort non-biohazardous cells.

  • Human samples must be sorted exclusively on the FACSAria II or FACSymphony S6 by Facility staff.
  • A copy of your Schedule F is required for any biohazardous materials brought into the core.

Contact Facility staff for training or questions.

The Facility Director is available to assist with any flow cytometry needs. To schedule a consultation, please call or email the Facility for an appointment.

First-Time User Overview

  1. Review the website for Facility rules and an overview of flow cytometry.
  2. Familiarize yourself with relevant protocols.
  3. Discuss your needs and feasibility with the Facility Director or staff.
  4. Schedule an appointment with the Facility (Contact Us). Facility staff will run your samples until you are fully trained.
  5. Observe sample analysis and familiarize yourself with the process.
  6. Analyze your data.
  7. Publish your results—become famous!