A priority mission of the Flow Cytometry Core Facility is to educate users in properly designing flow cytometry experiments, operate analytic flow cytometers, and analyze flow cytometry data.
All users who wish to operate the analyzers are required to attend a three part training course. To initiate facility training, please Create an account in iLab. Once you have created your iLab account, you can initiate a request for the Introductory Training followed by the Instrument-Specific Training.
- Part 1 (Part 1 (Introductory Training – access to WORK-FLOW‘s Flow Cytometry Essentials) serves to acquaint the users with the basics of how a flow cytometer works and, especially, what is going on inside “the box” when the user activates various functions. A basic understanding of how a flow cytometer works is critical to producing high quality data. We provide a UNC-specific module that includes important aspects of working in our FlowCore. You may Request Training in iLab to enroll. You will then receive an email with instructions on accessing the online training material.
- Part 2 (Instrument-Specific Training) is required for each different machine (Attune NxT, LSR II/LSRFortessa, iQue Screener PLUS, and ISX MkII) the user wishes to use. Users must be trained by Facility staff. Training by others in the user’s laboratory is forbidden. Prior to Attune NxT training, study the Attune NxT Quick Reference.
- Part 3 (Best Practices Seminar) can be taken before or after Part 2. This is a 1.5 hour seminar with Dr. Diz, Core director, on the best practices when designing, running, and analyzing your flow data. We encourage this to be a two-way discussion so we can help you with your flow at the very beginning! You are automatically signed up for this seminar when you request Part 1.
Your first acquisition run must be scheduled with your trainer. Once completed you will be granted iLab calendar access and after-hour core access.
Part 1 is offered on-demand and is valid for 1 year (once expired you can retake the training free of charge). To sign up please register in iLab and then “request service” for the Introductory Training under Request Services.
To schedule instrument training (Part 2), please contact us by email:
- Attune NxT Training: Janet Dow or Ayrianna Woody (study the Attune NxT Quick Reference)
- LSR II/LSRFortessa Training: Janet Dow or Ayrianna Woody
- iQue Screener PLUS Training: Janet Dow
- ImageStreamX MarkII Training: Janet Dow
Multiple training sessions will be planned for the user to cover the following:
- Principles of Cell Sorting
- Instrument set-up
- Experiment set-up
- Basic Troubleshooting
- Instrument Shutdown
Eligibility for training on the FACSAria III – All criteria must be met:
- Have attended the Introductory Training (see part 1 above).
- Have used a FACSDiva-based flow cytometer for the past six months at either UNC or an equivalent institution*.
- Have a need for at least 2 hours/week of non-biohazardous sorting.