Core Access
The UNC Flow Cytometry Facility is available to all UNC-CH investigators. The Facility is also available to investigators outside UNC – Duke, NC State and companies in the RTP. Contract work with institutions other than those above can be accommodated. Access is mostly based on a first-come, first-served basis.
- Complete the iLab Account Sign-up Form. If you are at UNC, be sure to select ‘University of North Carolina at Chapel Hill’ on the drop down menu as the institution rather than typing in ‘UNC’ so it will recognize our core.
- You will receive an email with account information and basic instructions to get started using the system once your account has been approved.
Sorting – All sorting instruments are run by the facility staff. The FACSAria III is available to trained users to sort independently non-biohazardous cells. Contact Evan Trudeau for training.
The MoFlo and MoFlo XDP are not available for sorting biohazard samples.
All human samples must be sorted on the FACSAria II or the FACSAria III.
We require a copy of your SCHEDULE F for any biohazardous materials brought into the core.
See also our Sorting Information.
Consultation – The Facility Director is available for consultation on any component and aspect of flow cytometry. Please call or email for an appointment.
First time users overview:
- Read the web site for rules and overview of flow cytometry.
- Read appropriate protocols.
- Talk to Facility Director or staff about your needs and whether we can do what you want.
- Contact the Facility for an appointment (Contact Us). We will run your samples until you are trained (see Training).
- Bring samples to Facility for analysis. Observe samples being run to familiarize yourself.
- Analyze data.
- Write your paper – get famous!!