The Molecular Biology Core
Director: Wanda K O’Neal, PhD, Research Associate Professor
Molecular biology has become an indispensable component of modern biological research programs. The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for the Marsico Lung Institute/UNC Cystic Fibrosis Center.
- The Core serves as a centralized resource for cloning and sequencing of genes.
- Isolation, storage, and analysis of high quality RNA, including RNA sequencing.
- RNA in situ hybridization using RNA Scope(R).
- Generation of transgenic mouse models.
- Development of methods for si/shRNA knockdown in airway cell models.
- Use of CRISR/cas9 mediated gene editing for airway models.
- Providing training in molecular biology.
Update: New Equipment in the Core: As part of the Molecular Therapy Core Center AARA equipment funding, the Molecular Biology Core provides the following equipment for core users: fluor chem Q, alpha innotech gel imager, nano drop, PCR machines, lightcycler 480II, ABI 7500 fast real-time PCR system, ABI quantstudio6 flex 96 and 384 well, tissue culture hood and incubator, and fume hood for RNA isolation.
|Dr. Wanda O’Neal’s Lab Personnel|
Above group photo from left to right: Kristen Wilkinson, Kristy Terrell, Lisa Jones, Rodney Gilmore, Allison Volmer, and Joy Chen.
Contact Information125 Mason Farm Road
Campus Box #7248
The University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
(919) 962 9866
Fax:(919) 966 6821