Training

A priority mission of the Flow Cytometry Core Facility is to educate users in properly designing flow cytometry experiments, operate analytic flow cytometers, and analyze flow cytometry data. 

Upcoming Lecture                                                  Training Fees


 All users who wish to operate the analyzers are required to attend a two part training course. To initiate facility training, please create an account in iLab.  Once you have created your iLab account, you can initiate a request for the Lecture training followed by the instrument training. 

  1. Part 1 (a 3 hour introductory training lecture) serves to acquaint the users with the basics of how a flow cytometer works and, especially, what is going on inside "the box" when the user activates various functions. A basic understanding of how a flow cytometer works is critical to producing high quality data.
  2. Part 2 (a 3 hour one-on-one instrument-specific training) is required for each different machine (CyAn, LSRII/LSRFortessa, iQue Screener PLUS, and ISX MkII) the user wishes to use. Users must be trained by Facility staff. Training by others in the user's laboratory is forbidden. Note: prior to training on the LSR II, please review the FACSDiva software tutorial.

Your first acquisition run must be scheduled with your trainer. Once completed you will be granted iLab calendar access and after-hour core access.

Part 1 is offered monthly (based on demand) and is valid for 2 years (once expired you can retake the lecture free of charge). To sign up for the next available introductory training lecture please register in iLab and then "request service" for the Introductory Training Lecture under Request Services.


To schedule instrument training (Part 2), please contact us by email

 

Sort Training

The UNC Flow Cytometry Core Facility will train users to sort independently non-biohazardous cells on the FACSAria III.

Multiple training sessions will be planned for the user to cover the following:

Eligibility for training on the FACSAria III - All criteria must be met:

  • Have attended the Introductory Flow Cytometry Training Lecture (see part 1 above).
  • Have used a FACSDiva-based flow cytometer for the past six months at either UNC or an equivalent institution*.
  • Have a need for at least 2 hours/week of non-biohazardous sorting.
  • Prior to training on the FACSAria III, please review the FACSDiva software tutorial.
* if a user has operated a similar sorter at another institution, the user will still be required to attend the Introductory Flow Cytometry Training Lecture and be trained by Core staff in order to assess proficiency and knowledge of the cell sorting procedures. Training time can be dramatically shortened if such proficiency is successfully demonstrated. If you have any questions related to your experience, would like more specifics, or are interested in starting training, please contact Evan Trudeau.

The time required for sort training will be based on each individual's experience and learning. Certification for sorting independently will be at the sole discretion of the Core staff. Please note that there will be a limited number of available training slots and requests will be dealt with on a first-come first-served basis.

Training for other aspects of conducting flow cytometry experiments will be undertaken in individual discussions with users. We are happy to provide consultation to Core users and PIs free of charge. Our goal is for you to be successful with your flow cytometry experiments. In addition, there is useful information in the form of protocols and tutorials available on the website (Resources).