Scott H Randell, PhD

Associate Professor

Specialty Areas: Airway Epithelial Stem Cells, Airway Innate Immunity and Response to Injury, mRNA regulation

Research Focus:

Identification of airway epithelial stem cells. Dr. Randell’s goals are to identify and isolate airway epithelial stem cells and to understand molecular mechanisms regulating airway epithelial cell proliferation and fate decisions.Research in Dr. Randell’s laboratory is currently focused on five areas where a greater knowledge of basic cell biology can be applied towards overcoming clinical lung disease problems:

  • Micro RNA Regulation of Human Airway Epithelial Phenotype- ARRA Award. The Randell Laboratory comprehensively determines the miRNA repertoire of human airway epithelial cells will tests the ability of miRNAs to alter cell structure and function. These studies will create a valuable database and will suggest novel treatments for lung disease.
  • Innate immunity in the airway. Dr Randell aims to better understand molecular mechanisms regulating airway epithelial adaptation to injury and chronic infection and to understand the relationship of adaptation to the pathogenesis of airway disease.
  • Post-lung transplant ischemia reperfusion injury and bronchiolitis obliterans syndrome. The Randell Laboratory is also examining basic mechanisms of lung injury relevant to transplantation and bronchiolitis obliterans syndrome, the leading cause of lung allograft failure.
  • Dr. Scott Randell also directs the UNC CF Center Tissue Procurement and Cell Culture Core, which provides primary and passaged human airway and alveolar epithelial cells, lung microvascular endothelial cells, media and expertise to UNC CF Center investigators and collaborators. Our facility has become a nationally and internationally recognized resource whose services are sought for collaboration, contract research, and training by academics, non-profit organizations, biotech and the pharmaceutical industry.
Figure 1. X-Y plane confocal microscopy of cultured human airway epithelial cells expressing green and red fluorescent protein.
Figure 1. X-Y plane confocal microscopy of cultured human airway epithelial cells expressing green and red fluorescent protein.
Figure 2. Human bronchial epithelial cells in culture, fixed with perflurocarbon OsO4 and prepared for high resolution light microscopy.
Figure 2. Human bronchial epithelial cells in culture, fixed with perflurocarbon OsO4 and prepared for high resolution light microscopy.
Figure 3. Frozen section of a human cystic fibrosis airway excised during lung transplantation, stained for mucus. Note the adhesion between the mucus in the lumen and the airway wall.
Figure 3. Frozen section of a human cystic fibrosis airway excised during lung transplantation, stained for mucus. Note the adhesion between the mucus in the lumen and the airway wall.
 
Selected Bibliography:
  1. Mitchel JA, Antoniak S, Lee JH, Kim SH, McGill M, Kasahara DI, Randell SH, Israel E, Shore SA, Mackman N, Park JA. IL-13 augments compressive stress-induced tissue factor expression in human airway epithelial cells. Am J Respir Cell Mol Biol. 2016 Apr;54(4):524-31. doi: 10.1165/rcmb.2015-0252OC. Epub 2015 Sep 25. PubMed PMID: 26407210.
  2. Park JA, Kim JH, Bi D, Mitchel JA, Qazvini NT, Tantisira K, Park CY, McGill M, Kim SH, Gweon B, Notbohm J, Steward R Jr, Burger S, Randell SH, Kho AT, Tambe DT, Hardin C, Shore SA, Israel E, Weitz DA, Tschumperlin DJ, Henske EP, Weiss ST, Manning ML, Butler JP, Drazen JM, Fredberg JJ. Unjamming and cell shape in the asthmatic airway epithelium. Nat Mater. 2015 Oct;14(10):1040-8. doi: 10.1038/nmat4357. Epub 2015 Aug 3. PubMed PMID: 26237129.
  3. Evans ES, Hackney AC, McMurray RG, Randell SH, Muss HB, Deal AM, Battaglini CL. Impact of acute intermittent exercise on natural killer cells in breast cancer survivors. Integr Cancer Ther. 2015 Sep;14(5):436-45. doi: 10.1177/1534735415580681. Epub 2015 Apr 14. PubMed PMID: 25873292.
  4. Fritzsching B, Zhou-Suckow Z, Trojanek JB, Schubert SC, Schatterny J, Hirtz S, Agrawal R, Muley T, Kahn N, Sticht C, Gunkel N, Welte T, Randell SH, Länger F, Schnabel P, Herth FJ, Mall MA. Hypoxic epithelial necrosis triggers neutrophilic inflammation via IL-1 receptor signaling in cystic fibrosis lung disease. Am J Respir Crit Care Med. 2015 Apr 15;191(8):902-13. doi: 10.1164/rccm.201409-1610OC. PubMed PMID: 25607238; PubMed Central PMCID: PMC4435455.
  5. Chand HS, Montano G, Huang X, Randell SH, Mebratu Y, Petersen H, Tesfaigzi Y. A genetic variant of p53 restricts the mucous secretory phenotype by regulating SPDEF and Bcl-2 expression. Nat Commun. 2014 Nov 27;5:5567. doi: 10.1038/ncomms6567. PubMed PMID: 25429397; PubMed Central PMCID: PMC4247165.
  6. Hogan BL, Barkauskas CE, Chapman HA, Epstein JA, Jain R, Hsia CC, Niklason L, Calle E, Le A, Randell SH, Rock J, Snitow M, Krummel M, Stripp BR, Vu T, White ES, Whitsett JA, Morrisey EE. Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function. Cell Stem Cell. 2014 Aug 7;15(2):123-38. doi: 10.1016/j.stem.2014.07.012. Review. PubMed PMID: 25105578; PubMed Central PMCID: PMC4212493.
  7. Cholon DM, Quinney NL, Fulcher ML, Esther CR Jr, Das J, Dokholyan NV, Randell SH, Boucher RC, Gentzsch M. Potentiator ivacaftor abrogates pharmacological correction of ΔF508 CFTR in cystic fibrosis. Sci Transl Med. 2014 Jul 23;6(246):246ra96. doi: 10.1126/scitranslmed.3008680. PubMed PMID: 25101886; PubMed Central PMCID: PMC4272825.
  8. Chen G, Korfhagen TR, Karp CL, Impey S, Xu Y, Randell SH, Kitzmiller J, Maeda Y, Haitchi HM, Sridharan A, Senft AP, Whitsett JA. Foxa3 induces goblet cell metaplasia and inhibits innate antiviral immunity. Am J Respir Crit Care Med. 2014 Feb 1;189(3):301-13. doi: 10.1164/rccm.201306-1181OC. PubMed PMID: 24392884; PubMed Central PMCID: PMC3977731.