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Measurements of Ciliary Function

The Marsico Lung Institute has an extensive array of technologies to measure ciliary function both in vitro and in vivo. Thus, it has available techniques for measurement of ciliary beat frequency in human and animal culture systems, measurements with high-speed videomicroscopy of ciliary beat form, ultrastructural measurement capacities to evaluate ciliary shaft structure, and the ability to perform proteomics of ciliary shaft proteins (Fig. 12, and Figs. 13-14).

Figure 12
Figure 12. Fractionation of ciliary proteins by SDS-PAGE. Each band was subjected to in-gel digestion and analyses by LC/MSE to identify and quantify the entire ciliary proteome.