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The Thermo Fisher Attune NxT is equipped with a 96/384 well plate Autosampler and 4 lasers (405, 488, 561, and 637nm) and can detect up to 14 fluorescent parameters. Its acquisition software is Attune NxT 2.6.

Attune V6 configuration

Attune NxT Ideal cell concentrations

Attune NxT User Guide

Quick Reference

Fluorophore Selection Guide (use for violet, blue, yelllow and red lasers)

Validated Plates for Autosampler

Attune NxT.jpg

Attune 1 located in Marsico Building – Room B018

Attune 2 located in Marsico Building – Room B021C

Samples containing viable BSL-2 agents (or samples that could contain such agents but whose status is unknown) may be run on the Attune 2.

We require a copy of your SCHEDULE F for any biohazardous materials brought into the core.

The investigator must comply with the following rules:

  • PPE – Lab coat, gloves, eye protection and closed toe shoes are to be used at all times.
  • Transport – Samples are to be transported to and from room B021C in an approved biosafety transport container.
  • Spills – If any sample is spilled in the room it is to be reported to Facility staff and cleaned up using bleach.
  • Disposal – No samples or other contaminated materials are to be disposed of down the sink.

Any contaminated materials should be discarded into the biohazard trash.
Left over samples are to be returned to the user’s lab using the approved transport container.

  • Cleaning – When finished running your samples, follow the cleaning procedure posted on the instrument.