Protocols

Genomics

Tufts NextGen sequencing primer (pdf)

Annealing Truseq adapters (doc)

Truseq adapter sequences (xlsx)

Kapa mRNA-seq library preparation (pdf)

NEB Next ChIP-Seq library preparation (doc)

Stat Quest (website)

Cloning

Alkaline lysis mini preps (docx)

Glycerol Stocks (docx)

Gibson Master Mix (docx)

Gibson Assembly (docx)

RNA

Gel extraction of RNA (docx)

Northern Blots (doc)

Nuclear Extraction (docx)

Pyrosequencing (docx)

Gentle extraction of RNA from chromatin-enriched fraction (docx)

DNA

Southern Blotting (doc)

Imaging

Fluorescent labeling of DNA probes for FISH (doc)

Immunofluorescence (docx)

RNA FISH/IF (docx)

IF/DNA/RNA FISH (docx)

Cell culture

Trophoblast stem cells (docx)

XEN cells (docx)

ESCs (doc)

epiSCs (docx)

293T transfection (doc)

Optimization of electroporation with Neon (doc)

Electroporation with Neon (doc)

Cellular Fractionation (doc)

TETRIS assay (docx)