Hyung-Suk Kim, PhD
My current interests include homeostatic regulation in hypertension and cardiac hypertrophy. To test human system in mice, we have generated animal models by using gene targeting of gene-knockout, gene duplication, 3’UTR or 5’UTR replacements. Decreased or increased protein levels by changed gene expression are valuable phenotypes for studying development of disease states.
Pandya K, Kim H.S., Smithies O. Fibrosis, not cell size, delineates beta-myosin heavy chain reexpression durin cardiac hypertrophy and normal aging in vivo. Proc Natl. Acad. Sci. USA 103: 16864-16869 (2006).
Lee, G., Makhanova, N., Caron, K., Lopez, M.L.S., Gomez, R.A., Smithies, O. and Kim, H.S. Homeostatic responses in the adrenal cortex to the absence of aldosterone in mice. Endocrinol.146: 2650-2656 (2005).
Kakoki, M., Tsai, Y-S., Kim, H.S., Hatada, S., Ciavatta, D.J., Takahashi, N., Arnold, L.W., Maeda, N. and Smithies, O. Altering the expression in mice of genes by modifying their 3’ regions. Developmental Cell, 6: 597-606 (2004).
Kim, H.S., Lee, G., John, S.W.M., Maeda, N. and Smithies, O. Molecular phenotyping for analyzing subtle genetic effects in mice: Application to an angiotensinogen gene titration. Proc. Natl. Acad. Sci.USA, 99: 4602-4607(2002).
Kim, H.S., Maeda, N., Oh, G.T., Fernandez, L.G., Gomez, R.A. and Smithies, O. Homeostasis in mice with genetically decreased angiotensinogen is primarily by an increased number of renin-producing cells. J. Biol. Chem. 274: 14210-14217 (1999).
Kim, H.S., Krege, J.H., Kluckman, K.D., Hagaman, J.R., Hodgin, J.B., Best, C.F., Jennett, J.C., Coffman, T.M., Maeda, N. and Smithies, O. Genetic control of blood pressure and the angiotensinogen locus. Proc. Natl. Acad. Sci. USA 92: 2735-2739 (1995).
Smithies, O. and Kim, H.S. Targeted gene duplication and disruption for analyzing quantitative genetic traits in mice. Proc. Natl. Acad. Sci. USA 91: 3612-3615 (1994).
Kim, H.S. and Smithies, O. Recombinant fragment assay for gene targeting based on the polymerase chain reaction. Nucleic Acids Res. 16: 8887-8903 (1988).