DPLM Faculty Profiles — Dr. Singh

Harsharan Singh

Harsharan Singh, MD

Professor

Office: 919-966-2421

E-mail: hsingh@med.unc.edu

Research Interests

Our research in the area of transplantation has  culminated in the characterization and development of a novel, non-invasive, diagnostic test to diagnose a major infectious complication post kidney transplantation known as polyomavirus nephropathy. This new diagnostic technique developed by me and my colleagues at UNC, called “Haufen testing”, avoids invasive biopsy procedures, and could potentially have profound implications for the care of kidney allograft recipients worldwide. The clinical impact of this novel discovery is now being confirmed in a multi-center prospective study with unrestricted funding from Astellas Pharma, US Inc. for which we are the lead investigative center.  We are the lead investigators with centers participating from the US, Canada, and Europe in developing an International Consensus Classification of Polyomavirus nephropathy. Funding for this work is currently under review with the ERA-EDTA (European Renal Association / European Dialysis and Transplantation Association).

Selected Publications

Nickeleit V and Singh HK:  Polyomaviruses and Disease: Is there more than viremia and viruria? Curr Opin Organ Transplant. 2015 Jun; 20(3):348-58.

Singh HK, Reisner H, Derebail VK, Kozlowski T, Nickeleit V. Polyomavirus Nephropathy: quantitative urinary polyomavirus-Haufen testing accurately predicts the degree of intra-renal viral disease. Transplantation 99(3): 609-615, 2015.

Nickeleit V, Singh HK. Vascular Pathology in Anatomic Pathology Board Review edited by Lefkowitch JH, 2nd ed. Saunders Elsevier, New York. 2014: 25-66.

Jennette JC, Singh HK. Renal Involvement in Polyarteritis Nodosa, Kawasaki Disease, Takayasu Arteritis, and Giant Cell Arteritis in Jennette JC, Olson JL, and Silva FG, D’Agati V (eds), Heptinstall’s Pathology of the Kidney, 7th Edition, Wolters Kluwer, Philadelphia, 2014, Chapter 17, pp. 715-738.

Sharma SG, Nickeleit V, Herlitz LC, de Gonzalez AK, Stokes MB, Singh HK, Markowitz GS, D'Agati VD. BK polyoma virus nephropathy in the native kidney. Nephrol Dial Transplant 28 (3):620-631, 2013.

Liapis G, Singh HK, Derebail VK, Gasim AMH, Kozlowski T, Nickeleit V. Diagnostic Significance of Peritubular Capillary Basement Membrane Multilaminations in Kidney Allografts: Old Concepts Revisited. Transplantation 94 (6):620-629, 2012.

Singh HK, Thompson BD, Nickeleit V. Viral Haufen are Urinary Biomarkers of Polyomavirus Nephropathy: New Diagnostic Strategies Utilizing Negative Staining Electron Microscopy. Ultrastruct Pathol 33 (5): 222-235, 2009.

Singh HK, Andreoni KA, Madden V, True K, Detwiler, R, Weck K, Nickeleit V. Presence of urinary Haufen accurately predicts polyomavirus nephropathy. J Am Soc Nephrol 20 (2): 416-427, 2009.

Singh HK, Andreoni K, Madden V, True K, Detwiler R, Weck K, Nickeleit V.  Presence of Urinary Haufen accurately predicts Polyomavirus Nephropathy.  Journal of the American Society of Nephrology 2009 20: 416-427.

Nickeleit V, Singh HK. Polyomavirus allograft nephropathy: clinico-pathological correlations. In “Chronic Allograft Failure: Natural History, Pathogenesis, Diagnosis and Management” Ahsan N (ed.), 1st edition, chapter 28, Landes Bioscience/ Eurekah.com (Austin, TX.), pp: 306-318, 2008.