DPLM Faculty Profiles — Dr. Singh

Harsharan Singh

Harsharan Singh, MD

Professor

Office: 919-966-2421

E-mail: hsingh@med.unc.edu

Research Interests

Our research in the area of transplantation has  culminated in the characterization and development of a novel, non-invasive, diagnostic test to diagnose a major infectious complication post kidney transplantation known as polyomavirus nephropathy. This new diagnostic technique developed by me and my colleagues at UNC, called “Haufen testing”, avoids invasive biopsy procedures, and could potentially have profound implications for the care of kidney allograft recipients worldwide. The clinical impact of this novel discovery is now being confirmed in a multi-center prospective study with unrestricted funding from Astellas Pharma, US Inc. for which we are the lead investigative center.  We are the lead investigators with centers participating from the US, Canada, and Europe in developing an International Consensus Classification of Polyomavirus nephropathy. Funding for this work is currently under review with the ERA-EDTA (European Renal Association / European Dialysis and Transplantation Association).

Selected Publications

Nickeleit V, Singh HK. Vascular Pathology in Anatomic Pathology Board  Review edited by Lefkowitch JH, 2nd ed. Saunders Elsevier, 2012; in press.

Volker Nickeleit, Karin True, Randal Detwiler, Tomasz Kozlowski, and Harsharan K. Singh.  Risk Assessment for Polyomavirus Nephropathy using Urine Cytology and the Detection of Decoy-Cells: Cheap and Efficient. Transplantation. in press.

Liapis G, Singh HK, Derebail VK, Gasim AMH, Kozlowski T, Nickeleit V.  Diagnostic Significance of Peritubular Capillary Basement Membrane Multilaminations in Kidney Allografts: Old Concepts Revisited. Transplantation. in press.

Saad R, Singh HK, Silverman JF. Paediatric tumours in Svante RO and Sterrett GF, eds. Fine Needle Aspiration Cytology, 5th edition. Elsivier, Ltd. New York. 2011:  227-286.

Elsheikh TM, Singh HK, Silverman JF. Fine Needle Aspiration Cytology of the Head and Neck.  In:  Surgical Pathology of the Head and Neck, Volume 1, Third Edition (Barnes L. Ed.). Informa Healthcare USA, Inc, New York. pp. 1-94. 2009.

Singh HK, Thompson BD, Nickeleit V.  Viral Haufen are Urinary Biomarkers of Polyomavirus Nephropathy: New Diagnostic Strategies Utilizing Negative Staining Electron Microscopy. Ultrastructural Pathology, 2009 Dec; 33: 222-235.

Derebail VK, McGregor JG, Colindres RE, Singh HK, Kshirsagar AV. The Case:  Acute Kidney Injury in a patient with P. Carnii pneumonia. Kidney Int. 2009 Apr; 75(8):865-6.

Singh HK, Andreoni K, Madden V, True K, Detwiler R, Weck K, Nickeleit V.  Presence of Urinary Haufen accurately predicts Polyomavirus Nephropathy.  Journal of the American Society of Nephrology 2009 20: 416-427.

Nickeleit V, Singh HK. Polyomavirus allograft nephropathy: clinico-pathological correlations. In “Chronic Allograft Failure: Natural History, Pathogenesis, Diagnosis and Management” Ahsan N (ed.), 1st edition, chapter 28, Landes Bioscience/ Eurekah.com (Austin, TX.), pp: 306-318, 2008.

Singh HK, Volmar KE, Elsheikh TM, Silverman JF:  The Diagnostic Utility of Fine Needle Aspiration Biopsy of Soft Tissue Sarcomas in the Core Needle Biopsy Era. Pathology Case Reviews, 12(1):  36-43, 2007.

Silverman JF, Elsheikh TM, Singh HK: The Role of Fine Needle Aspiration Cytology of the Breast in the Core Needle Biopsy Era.  Pathology Case Reviews 12(1): 44-47, 2007

Volmar KE, Singh HK, Gong JZ:  Fine Needle Aspiration Biopsy of Lymph Nodes in the Modern Era:  Reactive Lymphadenopathies. Pathology Case Reviews, 12(1):  27-35, 2007

Volmar KE, Singh HK, Gong JZ:  The Advantages and Limitations of the Role of Core Needle and Fine Needle Aspiration Biopsy of Lymph Nodes in the Modern Era: Hodgkin and non-Hodgkin Lymphomas and Metastatic Disease. Pathology Case Reviews,  12(1):  10-26, 2007

Elsheikh TM, Singh HK, Silverman JF:  Fine Needle Aspiration Cytology versus Core Needle Biopsy in the Evaluation of Thyroid and Salivary Gland Lesions. Pathology Case Reviews, 12(1):  3-9, 2007

Singh HK, Madden V, Shen YJ, Thompson D, Nickeleit V:  Negative Staining Electron Microscopy of Urine in the Evaluation of Polyomavirus Infections. Ultrastructural Pathology, 30: 329-338, 2006

Srivastava T, Garola RE, Singh HK:  Cell Cycle Regulatory Proteins in the Podocyte Cell in Idiopathic Collapsing Glomerulopathy (CGN) in Children.  Kidney International, Kidney Int. 2006 Aug; 70(3):529-35.